盐酸氨溴索减轻胃液误吸导致的大鼠急性肺损伤

Ambroxol hydrochloride alleviates acute lung injury induced by gastric juice aspiration in rats

目的 探讨盐酸氨溴索在大鼠误吸胃液后导致的急性肺损伤中的治疗保护作用。方法 将SD大鼠随机分为对照组、模型组(采用1.5 mL/kg, pH≈2.3的大鼠胃液经环甲膜穿刺构建误吸模型)及盐酸氨溴索干预组(30 mg/kg)。造模24 h后开始经尾静脉给药,每天1次,连续3 d。HE染色检测肺组织,肺湿干重比(W/D)值和动脉血气评估肺损伤程度,ELISA检测血清中IL-6、IL-10、TNF-α和C-反应蛋白(CRP)水平。结果 与对照组比较,模型组大鼠肺泡结构萎缩、间质水肿、炎性细胞渗出及透明膜形成,W/D值增高,pH、动脉血氧分压(pO)降低,动脉血二氧化碳分压(pCO)升高,血清炎性因子(IL-6、IL-10、TNF-α和CRP)水平升高(P<0.05)。与模型组比较,盐酸氨溴索干预组肺组织病理变化减轻,W/D值降低,pH、pO回升,pCO降低,血清炎性因子(IL-6、IL-10、TNF-α和CRP)水平回降(P<0.05)。结论 盐酸氨溴索能有效减轻大鼠误吸胃液所导致的急性肺损伤,具有较好的治疗和保护作用。

Objective To investigate the therapeutic and protective effect of ambroxol hydrochloride on acute lung injury caused by aspiration of gastric juice in rats. Methods SD rats were randomly divided into control group, model group(constructed by trans-cricothyroid membrane puncture with pH≈2.3 rat gastric juice, 1.5 mL/kg) and ambroxol hydrochloride intervention group(30 mg/kg). After 24 hours of modeling, the drug was administered through caudal vein once a day for 3 days. HE staining was used to detect lung tissue, lung wet to dry weight ratio(W/D) value and arterial blood gas analysis to evaluate the degree of lung injury, ELISA was used to detect IL-6, IL-10, TNF-α and C-reactive protein(CRP) levels in serum. Results Compared with the control group, the alveolar structure showed atrophy, interstitial edema, and inflammatory cell exudation. Hyaline membrane was formed and W/D value increased, pH and arterial oxygen pressure(pO) decreased, arterial carbon dioxide pressure(pCO) increased, and serum inflammatory factors(IL-6, IL-10, TNF-α and CRP) increased(P<0.05). Compared with the model group, the intervention reduced the pathological changes of lung tissue, W/D value decreased, pH and pOincreased, pCOdecreased, serum inflammatory factors(IL-6, IL-10, TNF-α and CRP) decreased(P<0.05). Conclusions Ambroxol hydrochloride can effectively reduce the acute lung injury in rats caused by gastric juice aspiration and has good therapeutic and protective effects.