宫内生长受限湖羊新生羔羊的血浆代谢组学研究

Metabolomics in Neonatal Lambs of Hu-sheep with Intrauterine Growth Retardation

湖羊是我国珍贵的肉毛兼用的绵羊品种,并具有繁殖率高和宜舍饲等特点。在生产中,往往因其多胎性易导致妊娠期湖羊营养不良,造成新生羔羊宫内生长受限(intrauterine growth retardation,IUGR)。应用代谢组学一维氢谱核磁共振技术(1H-nuclear magnetic resonance,1H-NMR)研究新生羔羊的血浆代谢谱。根据出生体质量筛选出14只湖羊新生羔羊,分为正常出生体质量组(normal birth weight,NBW)和IUGR组(n=7),采用1H-NMR分析其血液样品。将代谢谱数据进行统计分析,并对鉴定的代谢物进行表达差异分析以及代谢途径归属。NBW组和IUGR组新生羔羊血浆样品共获得32种代谢物,16种代谢物在2组间差异表达,IUGR组有10个代谢物含量显著低于NBW组(P<0.05),6个代谢物含量显著高于NBW组(P<0.05)。2组间差异显著的代谢通路主要为氨基酸、碳水化合物、脂类物质等代谢通路。应用基于1H-NMR的代谢组学及生物信息学分析方法,确定了IUGR新生羔羊与正常羔羊血液代谢物之间存在差异,为进一步深入研究IUGR的分子机制奠定了基础。

As a precious breed in China,Hu-sheep is a good duel-purpose breed both for mutton and wool,which have precociousness and hyper-prolificacy.Because of multiple pregnancy,it is easy to lead to malnutrition during pregnancy,resulting in intrauterine growth restriction(IUGR)of newborn lambs.In order to analyze the plasma metabolic profiles of neonatal lambs with IUGR and compare them with controls to define the metabolic patterns associated with IUGR,14 neonatal lambs of Hu-sheep were screened according to birth weight,and then allotted to two groups,normal birth weight(NBW)and IUGR groups(n=7).1H-nuclear magnetic resonance(1H-NMR)based metabolomics approach of the plasma samples collected from the two groups was obtained.The results was used to screen differential metabolites and analyze related metabolic pathways.The plasma samples of the NBW and IUGR groups were detected by1H-NMR,and 32 metabolites were obtained.16 metabolites were differentially expressed between the 2 groups.Compared with NBW group,the concentrations of 10 metabolites were significantly lower in IUGR group(P<0.05),and the concentrations of 6 metabolites were significantly upper(P<0.05).The significant metabolic pathways of those metabolites were amino acids,carbohydrates,and lipids pathways.This study indicated that1H-NMR based on metabolomics and bioinformatics analysis could be used to characterize changes in metabolomics between neonates lambs of IUGR and controls,which provided foundation for further in-depth molecular mechanism studies on IUGR.