TCAB1蛋白在喉鳞癌组织中的表达及与临床特征之间的关系

Expression of TCAB1 in laryngeal squamous cell carcinoma and its correlation with clinical features

目的 通过检测喉鳞癌组织中EB病毒(EBV)感染和TCAB1蛋白的表达情况,初步探讨TCAB1蛋白过表达在EBV感染阳性喉鳞癌组织中的作用机制。方法 收集2011—2018年宁夏医科大学总院(n=113)及上海市眼耳鼻喉科医院(n=12)的125份喉鳞癌及癌旁组织标本进行EBV基因检测及免疫组化TCAB1蛋白定性检测分析、喉鳞癌患者术后生存分析。结果 肿瘤发生部位多位于声门区(92/125,73.6%),病程多在一年之内(91/125,72.8%);EBV感染喉鳞癌的阳性率为61.6%(77/125),且喉鳞癌组织的分化程度、临床分期、肿瘤部位比较,差异无统计学意义(P> 0.05);TCAB1蛋白主要在喉鳞癌组织的细胞核内表达;TCAB1蛋白阳性率为78.4%(98/125),其与喉鳞癌的发生发展,差异有统计学意义(P<0.05),同时与EB病毒感染也有关(P <0.05),但TCAB1蛋白的表达与喉鳞癌患者的喉鳞癌组织的分化程度、临床分期、肿瘤部位比较,差异无统计学意义(P> 0.05);EBV感染、TCAB1蛋白的表达情况与喉鳞癌术后患者的预后比较,差异无统计学意义(P> 0.05),但肿瘤部位是喉鳞癌术后患者的预后独立危险因素。结论 本研究发现EBV感染及TCAB1蛋白表达均与喉鳞癌的发生发展密切相关,提示TCAB1基因参与了EBV感染喉鳞癌的发病过程。

Objective To preliminarily investigate the function mechanism of overexpression of Telomerase Cajal body protein 1(TCAB1) in Epstein-Barr virus(EBV)-positive laryngeal squamous cell carcinoma(LSCC) by detecting infection of EBV and expression of TCAB1 in LSCC. Methods A total of 125 cases of LSCC and adjacent specimens admitted to General Hospital of Ningxia Medical University(n=113) and Eye & ENT Hospital(n=12) of Shanghai City from 2011 to 2018 were collected for gene detection of EBV and qualitative immunohistochemical detection of TCAB1, and postoperative survival of patients with LSCC was analyzed. Results Most of the tumors were located in the glottic area(accounting for 92 in 125, which was 73.6%). And the course of disease was mostly within 1 year(accounting for 91 in 125, which was 72.8%). The positive rate of EBV infection in LSCC was 61.6%(accounting for 77 in 125), and there were no statistically significant correlations with differentiation degree of LSCC, clinical stage and tumor location(P > 0.05). TCAB1 was mainly expressed in the nucleus of LSCC, the positive rate of TCAB1 was 78.4%(accounting for 98 in 125), which was significantly correlated to the occurrence and development of laryngeal cancer(P < 0.05), and it was also correlated to EBV infection(P < 0.05). However,the expression of TCAB1 was not significantly correlated to the differentiation degree of LSCC, clinical stage and tumor location of patients with laryngeal cancer(P > 0.05). There were no statistically significant correlations with EBV infection, TCAB1 expression and the prognosis of postoperative patients with laryngeal cancer(P > 0.05).However, the location of tumor was an independent risk factor for the prognosis of postoperative patients with laryngeal cancer. Conclusion EBV infection and TCAB1 expression are both closely related to the occurrence and development of LSCC, suggesting that TCAB1 gene is involved in the pathogenesis of EBV infected LSCC.