摘要
Molecular phylogeny has revealed that sporotrichosis is caused by several Sporothrix species which differ in clinical behavior.The complex is embedded within Ophiostoma,a genus mainly comprising fungi that live in association with bark beetles,but differs by a high virulence towards humans and other mammals.The different ecology is corroborated by phylogenetic separation.The aim of the present study was to determine the validity of the rDNA internal transcribed spacer(ITS)region as a marker for diagnostics of species in the clinical group,using betatubulin sequences to calibrate species delimitations.The topology of the two gene trees was concordant,and all clinically relevant Sporothrix species could easily be recognized by means of the ITS region.An increased geographic sampling did not affected delimitation success in the clinical clade of the S.schenckii complex.
基金
Xun Zhou acknowledge financial support from China NSFC 31270062
Chongqing Science and Technology Commission cstc2011jjA10089
Anderson Rodrigues is a fellow and acknowledges the financial support of the Fundação de AmparoàPesquisa do Estado de São Paulo(FAPESP-2011/07350-1)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior(BEX 2325/11-0)。
