摘要
Thirty-six strains of endophytic Colletotrichum species were isolated from leaves of Bletilla ochracea Schltr.(Orchidaceae)collected from 5 sites in Guizhou,China.Seventeen different species,including 7 new species(namely C.bletillum,C.caudasporum,C.duyunensis,C.endophytum,C.excelsum-altitudum and C.guizhouensis and C.ochracea),8 previously described species(C.boninense,C.cereale,C.destructivum,C.karstii,C.liriopes,C.miscanthi,C.parsonsiae and C.tofieldiae)and 2 sterile mycelia were identified.All of the taxa were identified based on morphology and phylogeny inferred from multi-locus sequences,including the nuclear ribosomal internal transcribed spacer(ITS)region,partial genes ofβ-tubulin(TUB2),actin(ACT)and glyceraldehyde-3-phosphate dehydrogenase(GAPDH).Comprehensive morphological descriptions and illustrations are provided for new species.Our investigation indicates a high diversity of Colletotrichum species in B.ochracea.
基金
supported by NSFC(No.30960002 and No.31070020)
Guizhou Scientific and Technological Project,China(QianKeHe NY Zi(2010)No.3067).Gang Tao acknowledges China Postdoctoral Science Foundation(No.2011 M500421)
supporting his research at CAS.Yuanying Su and Dianming Hu are sincerely thanked for the technical assistance
We are very grateful to Dr.Roger G.Shivas,who gave valuable suggestions to this work during his academic visit to China(funded by NSFC 31110103906).
