响应面法优化大肠杆菌异源合成番茄红素

Optimization of heterogeneous lycopene synthesis in Escherichia coli using response surface methodology

番茄红素是一种C_(40)类胡萝卜素,具有强抗氧化性和提高免疫力等功效,已广泛应用于食品和化妆品等行业。为提高大肠杆菌异源生产番茄红素效率,筛选出最适大肠杆菌生产菌株Top10,采用单因素试验和响应面法优化发酵培养基,确定番茄红素生产最佳配方为LB培养基中添加果糖4.22 g/L,NH_(4)Cl 0.89 g/L和正十二烷15%(体积分数)。在此条件下,番茄红素得率达到29.3 mg/g DCW,比优化前提高了138%。实时荧光定量PCR分析表明,优化后番茄红素合成关键基因IspG和crtE表达比优化前显著增强(P<0.05),分别提高6.9倍和4.5倍。该研究通过宿主筛选和培养基优化显著提高了番茄红素合成效率,为大肠杆菌高效生产番茄红素奠定基础。

Lycopene,a C_(40)terpenoid compound,has strong anti-oxidation and immunity enhancement effects,which have been widely used in the food and cosmetics industries.To improve the efficiency of heterologous production of lycopene,this study screened Escherichia coli top10 as the suitable production strain and optimized fermentation medium by single factor experiment and response surface methodology.The optimal medium was medium with 4.22 g/L fructose,0.89 g/L NH_(4)Cl and 15%n-dodecane.Under this condition,the yield of lycopene reached 29.3 mg/g DCW,which was 138%higher than before optimization.Based on real-time fluorescent quantitative PCR,the expressions of key genes IspG and crtE for lycopene synthesis were significantly enhanced(P<0.05)after optimization,which increased by 6.9 times and 4.5 times,respectively.This study significantly improved the yield of lycopene through host screening and medium optimization,laying the foundation for the efficient production of lycopene in E.coli.