绵羊HSPA1L基因的生物信息学分析

Bioinformatic Analysis of HSPA1L Gene in Sheep

为了解绵羊热休克蛋白1L(heat shock protein family A member 1 like,HSPA1L)基因及其编码产物的基本结构和生物学特征,给绵羊的抗热应激和免疫等方面的研究提供依据,利用生物信息学数据库及软件对绵羊HSPA1L基因进行生物信息学分析。结果表明,该基因最大长度的ORF有1926 bp,编码641个氨基酸序列。其编码的蛋白质分子式为C_(3094)H_(4985)N_(859)O_(969)S_(20),理论等电点为5.89,不稳定指数为32.56。该蛋白无信号肽和跨膜结构,为非分泌性蛋白,主要在细胞质中发挥生物学作用。无规则卷曲是构成该蛋白二级结构和三级结构的主要方式。

In order to understand the basic structure and biological characteristics of the heat shock protein family A member 1 like(HSPA1L)gene and its coding products in sheep,the bioinformatic database and software were used to conduct bioinformatic analysis of the HSPA1L gene in sheep.The results showed that the ORF of the maximum length of the gene was 1926 bp,encoding 641 amino acid sequences.The protein molecular formula it encoded was C_(3094)H_(4985)N_(859)O_(969)S_(20),the theoretical isoelectric point was 5.89,and the instability index was 32.56.This protein with no signal peptides and transmembrane structure,was a non-secretory protein,and plays a biological role mainly in the cytoplasm.Irregular curling was the main way of constituting the secondary and tertiary structure of the protein.