miRNA-148b-3p targeting SOCS3 inhibits macrophage M2 polarization by JAK2/STAT3 pathway in immune thrombocytopenia

Aberrant expression of miRNAs is significantly correlated with the occurrence of immune thrombocytopenic purpura(ITP).The immune imbalance of M1/M2 macrophage contributes to the development of ITP.However,the role of miR-148b-3p in macrophage phenotype imbalance remains unknown in ITP.In this study,we aimed to explore whether miR-148b-3p inhibits M2 macrophage polarization in ITP and to investigate the underlying mechanism.Peripheral blood from 22 ITP patients were collected,and real-time PCR confirmed that miR-148b-3p was upregulated and Western blot analyses detected the expression of SOCS3 was down-regulated.Subsequent dualluciferase reporter gene assay indicated that miR-148b-3p could bind to SOCS3.Furthermore,we found significant correlation between miR-148b-3p expression and platelet count.Applying gain and lose the function experiments of miR-148b-3p and SOCS3,we demonstrated that suppression of miR-148b-3p or up-regulation of SOCS3 promoted macrophage M2 polarization by inhibiting JAK2/STAT3 pathway.Together,our findings demonstrate that that miR-148b-3p targeting SOCS3 inhibits M2 macrophage polarization via JAK2/STAT3 signaling in ITP.