大鼠卒中后抑郁模型评价的网状Meta分析

A Network Meta-analysis of the Evaluation of Post-stroke Depression Models in Rats

目的 通过系统评价方法比较两种目前最常用的大鼠卒中后抑郁模型的制备方法的差异,为大鼠卒中后抑郁模型的选择与应用提供依据。方法 通过计算机检索维普、万方、知网、Sinomed、Pubmed、WOS、Embase等中英文数据库,搜集所有关于大鼠卒中后抑郁模型的文献,检索时限自建库到2020年8月22日。由两位研究人员独立进行文献筛选和数据提取,通过动物实验系统评价研究中心(the Systematic Review Centre for Laboratory animal Experimentation, SYRCLE)动物实验偏倚风险评估工具对文献质量进行评价,运用Stata 10.0软件进行网状Meta分析。结果 最终纳入51项研究,共998只大鼠。其中,在大鼠脑缺血后给予慢性温和不可预知性应激(chronic unpredictable mild stress, CUMS)干预的研究42项,共809只大鼠;采用慢性束缚应激(chronic restraint stress, CRS)干预的研究9项,共199只大鼠。两种造模方式在体重指标上的有效性排序为:CRS型>CUMS型,并且均自干预后14 d开始至28 d出现体重的显著降低(P<0.05);在糖水偏好指标上从高到低的排序为:CUMS型>CRS型,前者自干预后7 d开始出现糖水偏好率的显著降低(P<0.05),而后者自干预后14 d开始出现降低。此外,在旷场实验中,CUMS型自干预7 d出现显著的差异(P<0.05),而CRS型自14 d开始差异具有显著性(P<0.05)。结论 本研究结果表明,在大鼠脑缺血后给予CUMS干预较给予CRS干预更易产生抑郁样的行为表现。

Objective In order to provide a basis for the selection and application of the model of post-stroke depression in rats, the differences between the two most commonly used method for the preparation of the model of post-stroke depression in rats were compared by systematic evaluation.Method We searched Chinese and English databases including VIP, Wanfang, CNKI, Sinomed, PubMed, WOS, EMBASE on line, and collected all literatures about the post-stroke depression model in rats. Time limit was set since the establishment of the database to August 22, 2020. Literature screening and data extraction were conducted by two researchers independently. The quality of literatures was evaluated using the Systematic Review Centre for Laboratory animal Experimentation(SYRCLE) animal experiment bias risk assessment tool, and reticulation meta-analysis was performed by Stata10.0 software.Result A total of 998 rats were included in 51 studies. Among them, 809 rats received chronic unpredictable stress(CUMS) in 42 studies after cerebral ischemia. While the chronic binding stress(CRS) were carried out in of the rest of 9 studies including 199 rats. In terms of body weight, the availability of the two models was followed as CRS and CUMS, both of them showed significant weight loss since post-stress 14 d to 28 d(P<0.05). However, for the sucrose preference rate, CUMS was superior to CRS, the former displayed significant decrease from post-stress 7 d(P<0.05), while the latter began at post-stress 14 d. Furthermore, CUMS model showed a significant difference from post-stress 7 d in the open field test(P<0.05), while CRS model appeared after 14 d of intervention(P<0.05).Conclusion The present study showed that rats received CUMS after cerebral ischemia was easier to produce depression-like behavior than the intervention of CRS.