姜内生细菌鉴定及对储藏期梨黑斑病菌丝抑制作用初探

Identification of Ginger Endophytic Bacteria and Preliminary Study on Inhibition of Pear Black Spot Mycelia in Storage Period

实验采用对峙培养法,对姜果实内分离出来的6种内生细菌进行了其对贮藏期梨黑斑病菌菌丝的拮抗实验研究。结果表明:经初筛和复筛,4种姜内生细菌J_(1)、J_(2)、J_(4)和J_(5)对储藏期梨黑斑病病菌均有拮抗作用。复筛第5天时拮抗率分别是6.20%、18.22%、54.19%和12.79%;其中J_(4)的拮抗作用最为明显。在第7天时拮抗作用有所变化,拮抗率分别为7.15%、1.73%、53.46%和6.49%,但J_(4)的抑制作用依旧最为明显,J_(4)内生菌处理的梨黑斑病菌菌落直径始终与对照及其他处理间存在显著性差异,且J_(4)的抑制作用保持稳定。通过形态观察、生理生化实验和16S rDNA序列分析,将J_(4)菌株鉴定为枯草芽孢杆菌。实验结果为姜内生细菌J_(4)的开发利用提供了很好的基础理论研究。

In the experiment,six kinds of endophytic bacteria isolated from ginger fruit were antagonized by the sputum culture method,and the antagonistic effect on the mycelium of P.stipitis was studied.The results showed that the four ginger endophytic bacteria J_(1),J_(2),J_(4) and J_(5) have antagonistic effects on the storing pear black spot disease during the initial screening and rescreening.The antagonistic rate at the 5th day of rescreening were 6.20%,18.22%,54.19%and 12.79%,respectively.The antagonistic effect of J_(4) was the most obvious.At the 7th day,the antagonism changed,and the antagonism rates were 7.15%,1.73%,53.46%and 6.49%,respectively.However,the inhibitory effect of J_(4) was still the most obvious.The colony diameter of P.elegans treated with J_(4) endophyte had a significant difference with the control and other treatments respectively,and the inhibition of J_(4) remains stable.The J_(4) strain was identified as Bacillus subtilis by morphological observation,physiological and biochemical experiments and 16S rDNA sequence analysis.The experimental results provided a good basic theoretical study for the development and utilization of ginger endophytic bacteria J_(4).