金花茶UPLC指纹图谱的建立及亲缘关系分析

Analysis of UPLC fingerprint and genetic relationships of different Sect.Chrysantha

目的建立不同种类金花茶UPLC指纹图谱,结合其表型特征和化学成分差异分析其亲缘关系,为金花茶品种分类和应用提供依据。方法采用UPLC法建立22种金花茶的指纹图谱,采用中药色谱指纹图谱相似度评价系统(2012版)对金花茶UPLC指纹图谱进行相似度评价,并采用SPSS24.0软件进行主成分分析、聚类分析。结果选取了3个色谱共有峰,指认了11个已知化学成分。不同种金花茶共有峰相似度在0.247~0.740。主成分分析筛选出弄岗、柠檬黄、龙州、陇瑞、细叶、隆安、顶生、天峨、中东、毛瓣10种金花茶的化学成分含量较高。聚类分析结果显示龙州亚类单独聚为一类;凹脉、顶生、天峨、显脉、弄岗、倒卵叶、柠檬黄、薄叶、中东、东兴、防普金花茶聚为一类;陇瑞、龙州亚种、细叶金花茶为一类;淡黄、扶绥、毛瓣、四季、恩城、隆安、武鸣金花茶聚为一类。结论金花茶种类繁多,不同种金花茶所含化学成分存在较大差异,结合表型特征,根据成分差异可基本获得种间亲缘关系。

Objective To establish of UPLC fingerprints of different kinds of Camellia nitidissima,and analyze the genetic relationship combined with its phenotypic characteristics and chemical composition differences,so as to provide the basis for the classification and application of Camellia nitidissima varieties.Methods The fingerprints of 22 batches of Camellia nitidissima were established by UPLC gradient elution,and the similarity evaluation of the UPLC fingerprints of Camellia nitidissima medicinal materials was carried out using the traditional Chinese medicine chromatographic fingerprint similarity evaluation system(2012 version),and SPSS24.0 software was used for clustering and PCA analysis.Results Three common chromatographic peaks were selected,and 11 known chemical constituents were identified.The common peak similarity of different kinds of C.nitidissima ranged from 0.247—0.740.Principal component analysis screened out GNG,GNMH,GLZ,GLR,GXY,GLA,GDS,GTE,GZD,GMB of 10 kinds of Camellia nitidissima with relatively high chemical components.The results of cluster analysis showed that the subcategories of C.Long zhouensis were clustered into one group alone.GAM,GDS,GTE,GXM,GNG,GDLY,GNMH,GBY,GZD,GDX,GFP were clustered into one group.GLR,GLZYZ and GXY clustered into one group.GDH,GFS,GMB,GSJ,GEC,GLA,GWM were clustered into one group.Conclusion There are many kinds of C nitidissima,and the chemical components contained in different kinds of C.nitidissima are quite different.According to the phenotypic characteristics and the composition differences,their interspecies genetic relationship can be basically obtained.