中药复方配伍理论下生血宝复方化学成分溶出规律研究

Study on dissolution law of chemical constituents of Shengxuebao compound under compatibility theory of TCM compound

目的通过检测7种配伍及7个单味组成药的单个化学成分与总响应成分的溶出量及变化情况,探讨生血宝复方配伍理论背后的科学内涵。方法根据生血宝复方中君臣佐使组方规律将各单味药重新进行配伍分组,建立16种成分(原儿茶酸、原儿茶醛、红景天苷、芍药苷、毛蕊异黄酮苷、二苯乙烯苷、木犀草苷、毛蕊异黄酮、特女贞苷、白藜芦醇、木犀草素、蟛蜞菊内酯、芒柄花素、旱莲苷A、大黄素、大黄素甲醚)同时含量测定的超高效液相色谱(UPLC)-二极管阵列检测器(PDA)方法学,并运用总量统计矩等方法进行定性定量分析。结果7种配伍分组(君、臣、佐使、君加臣、君加佐使、臣加佐使、复方)16个成分的总溶出量前3位的是E(君+佐使)、复方组、C(佐使),分别为2.9563、2.8846、2.0072 mg/g,与复方指纹图谱相似度最高前3位是D(君+臣)、B(臣)和墨旱莲,其值分别为0.767、0.754、0.711。结论从中药化学成分溶出规律角度探讨了中药复方配伍理论科学内涵,研究结果揭示了生血宝复方中各成分的溶出度受复方配伍规律的影响与支配,同时也为生血宝复方制剂的现代化研究奠定了基础。

Objective The scientific connotation behind the compatibility theory of the Shengxuebao compound was explored by detecting the dissolution and changes of a single chemical component and the total response component of seven compatibilities.Methods According to the prescription rules of monarch and minister assistants in the Shengxuebao compound,the single herbs were regrouped.The UPLC-PDA method was established for the simultaneous determination of 16 components(protocatechuic acid,protocatechuic aldehyde,salidroside,paeoniflorin,calycosin,stilbene glycoside,luteolin,calycosin,ligustrum lucidum glycoside,resveratrol,luteolin,wedelolactone,formononetin,ecliptin A,emodin and physcion)and the total statistical moment method was used for qualitative and quantitative analysis.Results The top three of the total dissolution of 16 components in 7 compatibility groups(monarch,minister,assistant,monarch plus minister,monarch plus assistant,minister plus assistant and compound)were E(monarch plus assistant),compound and C(assistant),which were 2.9563,2.8846 and 2.0072 mg/g,and the top three fingerprints with the highest similarity to the compound fingerprints were D(monarch+minister),B(minister)and Eclipta prostrate,and their values were 0.767,0.754 and 0.711.Conclusion The scientific connotation of the compatibility theory of traditional Chinese medicine compounds was discussed from the perspective of the dissolution law of chemical components of traditional Chinese medicine.The research results revealed that the dissolution of each component in the Shengxuebao compound was affected and controlled by the compatibility law of the compound,and laid the foundation for the modernization of Shengxuebao compound preparation.