藜芦bHLH转录因子分析鉴定

Analysis of Veratrum nigrum b HLH transcription factor based on transcriptome

目的基于转录组数据,利用生物信息学方法鉴定藜芦Veratrum nigrum bHLH转录因子基因家族,并作初步分析。方法通过隐马可夫模型从藜芦转录组序列中筛选bHLH基因,并使用ExPASy、MEME等在线网站及MEGA、TBtools等软件对藜芦bHLH进行理化性质、保守基序等可视化分析。结果在藜芦转录组中共鉴定到72条bHLH转录因子,VnbHLH转录因子氨基酸数目为105~692,等电点介于4.69~11.47,相对分子质量为11071.2~77795.3,亚细胞定位分析显示VnbHLH均定位于细胞核中;系统发育分析将VnbHLH转录因子分为6个亚族,VnbHLH在不同组织中表达差异显著,Vn_54312、Vn_14037在根中表达较高,推测其可能参与了藜芦植物根部某些生物碱的合成。结论根据转录组数据分析了药用植物藜芦中的bHLH转录因子基因家族,有助于下一步对藜芦bHLH转录因子家族功能预测及验证。

Objective To identify and analyze the Veratrum(Veratrum nigrum)bHLH transcription factor gene family of Veratrum veratrum by bioinformatics based on transcriptome data.Methods The bHLH gene was screened from the transcriptional sequence of Veratrum by hidden Markov model,and the physical and chemical properties and conserved motif of Veratrum bHLH were visually analyzed by online websites such as ExPASy and MEME and software such as MEGA and TBtools.Results A total of 72bHLH transcription factors were identified in Veratrum transcriptome.The amino acid number of VnbHLH transcription factors was 105—692,the isoelectric point was 4.69—11.47,and the relative molecular weight was 11071.2—77795.3.Subcellular localization analysis showed that VnbHLH were all located in the nucleus.According to phylogenetic analysis,VnbHLH transcription factors were divided into six subgroups.The expression of VnbHLH was significantly different in different tissues,while Vn_54312 and Vn_14037 were highly expressed in roots.It is speculated that they may be involved in the synthesis of some alkaloids in the roots of Chenopodium.Conclusion The analysis of bHLH transcription factor gene family in V.nigrum based on transcriptome data is helpful to predict and verify the function of bHLH transcription factor family in V.nigrum.