摘要
目的探讨microRNA-335-5p对人软骨细胞中WISP3的调节作用,研究microRNA-335-5p在骨性关节炎(OA)中的发病机制。方法通过分离并提取小鼠软骨细胞,分别转染microRNA-335-5p mimics、mimics NC、inhibitor、inhibitor NC质粒,通过qRT-PCR及Western Blotting检测各组中WISP3的表达水平。结果与阴性对照组相比,microRNA-335-5p过表达下调了WISP3的mRNA和蛋白水平的表达,相反microRNA-335-5p低表达上调WISP3的mRNA和蛋白水平的表达。结论microRNA-335-5p能抑制WISP3的mRNA转录及蛋白合成,可能通过靶向软骨细胞中的WISP3对OA的发生及发展起到潜在的作用。
Objective To investigate the regulatory effect of microRNA-335-5p on human chondrocytes WISP3 and the pathogenesis of miR-335-5p in OA.Methods Removal of articular cartilage from traumatic patients,separating and extracting chondrocytes,the expression level of WISP3 in each group was detected by qRT-PCR and Western Blotting after transfection of microRNA-335-5p mimics,mimics NC,inhibitor,inhibitor NC.Results Compared with the negative control group,microRNA-335-5p overexpression downregulated mRNA and protein expression levels of WISP3,on the contrary,the low expression of microRNA-335-5p up-regulated the expression of mRNA and protein in WISP3.Conclusion MicroRNA-335-5p can inhibit mRNA transcription and protein synthesis of WISP3,microRNA-335-5p plays a potential role in the pathogenesis and development of OA by regulating WISP3 in chondrocytes.
作者
黄殿华
李煜
陈顺有
潘源城
林然
卢育南
HUANG Dianhua;LI Yu;CHEN Shunyou;PAN Yuancheng;LIN Ran;LU Yunan(Fuzhou Second Hospital Affiliated to Xiamen University,Third Clinical Medical College of Fujian Medical University,Fujian Provincial Clinical Medical Research Center for First Aid and Rehabilitation in Orthopaedic Trauma,Fuzhou Trauma Medical Center,Fuzhou 350007,China)
出处
《中国医药指南》
2022年第22期73-76,共4页
Guide of China Medicine
基金
福州市科技计划项目(2019-SZ—22)
福建省创伤骨科急救与临床医学中心项目(2020Y2014)
福州市创伤医学中心项目(2018080303)。
