加温加压输血仪器核心性能检测的实验研究

Experimental study on core performance test of the heating and pressurizing Blood Transfusion Apparatus

目的检测自主研发加温加压输血仪器(输血/输液速度和温度可控)的核心性能。方法使用透析参考仪表检测并比较室温下生理盐水和悬浮红细胞实际流速;检测生理盐水(初始温度为(10±0.5)℃和(20±0.5)℃)在36℃、38℃、40℃和42℃预设加温条件下,流速达50 mL/min、100 mL/min、150 mL/min和200 mL/min时的输出温度;检测2~6℃冷藏悬浮红细胞在42℃预设加温条件下,流速为50 mL/min、100 mL/min、150 mL/min和200 mL/min时的输出温度,比较加温加压前后游离血红蛋白(free hemoglobin,FHb)含量、钾离子浓度及血常规差异。结果生理盐水和悬浮红细胞的实际流速无明显差异(P>0.05);加温加压输血仪器可使起始温度为(10±0.5)℃和(20±0.5)℃的生理盐水输出温度达到31~34℃。在42℃不同流速条件下,4℃冷藏悬浮红细胞的输出温度分别为(40.8±0.3)℃、(36.4±0.2)℃、(34.7±0.9)℃和(32.8±0.4)℃,高于初始温度(F=878.641,P=0.000,P<0.05)。加温加压后悬浮红细胞上清中FHb含量显著升高(P<0.05),但仍符合国家标准(≤0.72 g/L);钾离子浓度无明显升高;HGB、HCT、MCV、MCH差异无统计学意义。结论加温加压输血仪器显示了较佳的加温加压效果,对悬浮红细胞的溶血影响较小。

Objective To investigate the core performance of the self-developed heating and pressurizing blood transfusion apparatus(transfusion speed/temperature controllable).Methods We compared the flow rate between normal saline(NS)and suspended red blood cells(sRBCs)at room temperature by using the IBP Medical,detected the output temperatures of NS(initial temperatures of(10±0.5)℃and(20±0.5)℃)under the heating conditions of 36℃,38℃,40℃and 42℃and flow rates of 50 mL/min,100 mL/min,150 mL/min and 200 mL/min by a digital thermometer.We also detected the output temperature of sRBCs refrigerated at 2~6℃under the heating condition of 42℃and the flow rate of 50 mL/min,100 mL/min,150 mL/min and 200 mL/min,and compare the differences before and after heating and pressurizing of free hemoglobin(FHb),potassium ion concentration and blood routine.Results There was no significant difference between the actual flow rate of NS and sRBCs(P>0.05).The output temperature of NS with initial temperature of(10±0.5)℃and(20±0.5)℃could reach 31~34℃by heating and pressurizing blood transfusion apparatus.Under the conditions of different flow rates at 42℃,the output temperatures of refrigerated sRBCs at 4℃were(40.8±0.3)℃,(36.4±0.2)℃,(34.7±0.9)℃and(32.8±0.4)℃,which were higher than the initial temperature(P<0.05).After heating and pressurization,the FHb content in the supernatant of sRBCs increased significantly(P<0.05),but still met the national standard(≤0.72 g/L);There was no statistical difference in potassium ion concentration,HGB,HCT,MCV and MCH indexes before and after heating and pressurization.Conclusion The apparatus showed a good heating and pressurizing effect and little influence on hemolysis of sRBCs.