CRISPR-Cas12a在食源性致病菌检测中的应用

Application of CRISPR-Cas12a in the detection of foodborne pathogens

食源性致病菌快速检测方法对食源性疾病的高效预防和控制具有重要意义。CRISPR(clustered regularly interspaced short palindromic repeats)及相关蛋白(CRISPR-associated protein,Cas)构成的CRISPR-Cas系统是一种强大的基因编辑工具,基于其对靶标核酸的特异性识别及切割活性,应用于食源性致病菌检测中,已成为快速检测方法研究的热点。CRISPR-Cas12a检测技术具有特异性强、灵敏性高的优点,在食源性致病菌的检测中有着巨大的应用前景。本文主要介绍了CRISPR-Cas12a的作用机制,重点综述了CRISPR-Cas12a结合多种核酸扩增技术在食源性致病菌检测中的研究进展,进一步讨论了CRISPR-Cas12a在致病菌检测中存在的问题和不足,对未来的研究前景进行了展望,以期为更好地开发准确、快速灵敏的食源性致病菌检测技术提供参考和依据。

Rapid detection methods for foodborne pathogens are important for efficient prevention and control of foodborne diseases.Clustered regularly interspaced short palindromic repeats(CRISPR)and its associated proteins constitute the CRISPR-Cas system,a powerful gene editing tool,which has become a hot spot for research on rapid detection methods based on its specific recognition and cleavage activity of target nucleic acids when applied to the detection of foodborne pathogens.CRISPR-Cas12a detection technology has the advantages of high specificity and sensitivity,and has great application in the detection of foodborne pathogens.This paper introduced the mechanism of action of CRISPR-Cas12a,focused on the research progress of CRISPR-Cas12a combined with various nucleic acid amplification techniques in the detection of foodborne pathogens,further discussed the problems and shortcomings of CRISPR-Cas12a in pathogens detection,and provided an outlook on the future research prospects.In order to provide a reference and basis for better development of accurate,rapid and sensitive detection techniques for foodborne pathogens.