纺锤状羟基氧化铁@聚吡咯复合纳米粒子的制备及其光热治疗肿瘤应用

Preparation of spindle-shaped hydroxyl ferric oxide@polypyrrole composite nanoparticles and its application in photothermal therapy of tumors

肿瘤是世界上死亡率最高的疾病,研制新型肿瘤药物也是一项极具开发性的研究。首先制备纺锤状羟基氧化铁纳米粒子(FeOOH NPs)并作为模板,与吡咯(Py)聚合得到纺锤状羟基氧化铁@聚吡咯(FeOOH@PPy)复合纳米粒子。采用FTIR、DLS、TEM和UV-vis等测试方法对FeOOH@PPy进行了结构、性质表征,通过光热转换实验证明了该复合纳米粒子具有优异的光热转换性能,探究了复合纳米粒子的生物相容性以及肿瘤治疗中的应用潜力。结果表明,所得到的复合纳米粒子平均粒径100 nm左右,且分布均一,粒子呈纺锤状结构,在水溶液中具有良好的稳定性。FeOOH@PPy复合纳米粒子可以吸收808 nm的近红外光,并将其转化为足够的热量,使肿瘤细胞凋亡;在为期14天的4T1细胞的小鼠模型体内治疗实验中,FeOOH@PPy复合纳米粒子治疗组表现出优异的治疗肿瘤效果,组织分析表明FeOOH@PPy复合纳米粒子对小鼠正常组织无明显影响,具有作为光热治疗剂的潜力。

Cancer is a disease with the highest mortality rate in the world, developing new cancer drugs is also a very exploratory research. In this paper, spindle-shaped iron hydroxyl oxide(FeOOH NPs) was firstly prepared as a nanocarrier to polymerise pyrrole(Py) to get iron hydroxyl oxide @polypyrrole(FeOOH@PPy) composite nano-material. The structure and properties of FeOOH@PPy were characterized by FTIR, DLS, TEM and UV-vis, etc. The photothermal conversion experiments proved that the composite nanoparticles had excellent photothermal conversion properties, and the biocompatibility and application potential of the composite nanomaterial in tumor therapy were explored. The results show that the average particle size of the obtained nanoparticles is about 100 nm,and has good stability in the aqueous solution. The results of the photothermal performance test show that FeOOH@PPy composite nanoparticles can absorb 808 nm near-infrared light and convert it into enough heattotrigger the apoptosis of tumor cells. In a 14 days treatment on 4T1 breast cancer mouse model, FeOOH@PPy composite nanoparticles show excellent antitumor effect. Tissue analysis show that the composite nanoparticles have no significant effect on normal tissues of mice, suggesting that the composite nanoparticles have the potential as photothermal therapy agent.