茶树冷休克结构域蛋白基因CsCSP克隆与表达分析

Cloning and expression analysis of cold shock domain protein genes(CsCSPs)in Camellia sinensis

植物冷休克结构域蛋白(cold shock domain protein,CSP)是一种DNA/RNA结合蛋白,在植物生长发育和抗逆响应中具有重要作用。本研究从茶树(Camellia sinensis)基因组中鉴定并克隆获得4个CsCSP基因,分别命名为CsCSP1~4。系统进化分析显示这些基因分为2个亚家族。共线性分析发现CsCSP1~3中有3个基因复制事件发生。组织特异性分析发现,CsCSP在茶树不同组织中均有表达,其中,CsCSP1在茶树不同组织中表达丰度均高于其他3个基因。非生物胁迫条件下,CsCSP在处理不同时间点差异表达,其中,CsCSP2的表达受低温和盐胁迫处理显著抑制,而CsCSP4在处理不同时间点受不同处理诱导上调表达。脱落酸或赤霉素处理条件下,CsCSP在处理不同时间点被诱导上调表达。氧化胁迫条件下,CsCSP2~4均被诱导上调表达。冷驯化期间,除CsCSP3外,其他3个CsCSP在不同抗寒茶树品种冷驯化期间均表现出相反的表达模式。综合表明,CsCSP在茶树抗逆响应和生长发育过程中发挥重要作用。本研究为深入探究CsCSP的功能提供理论基础。

As a type of DNA/RNA-binding protein,cold shock domain protein(CSP)plays important roles in plant growth,development and various stress responses.Herein,4 CsCSPs genes,CsCSP1–4,were identified and cloned from Camellia sinensis.Phylogenetic analysis result shows that CsCSPs are clustered into two subgroups.Collinearity analysis result shows that three gene duplication events were occurred among CsCSP1–3.Tissue-specific analysis result shows that all CsCSPs were expressed in various tea plant tissues,and CsCSP1 showed the highest transcription abundances in all detected tissues than the other three CsCSPs.Under different abiotic stress conditions,CsCSPs were differentially expressed at different treatment time points.Among them,CsCSP2 transcript was gradually decreased under both cold and salt stress treatments,while CsCSP4 was induced by different abiotic stresses at different treatment time points.Besides,the expression of all CsCSPs were up-regulated by exogenous abscisic acid or gibberellin treatment at different treatment time points.Moreover,the expression levels of CsCSP2–4 were increased under oxidative stress condition.Furthermore,with the exception of CsCSP3,the other three CsCSPs showed different expression profiles in different cold-resistant tea plant cultivars during cold acclimation periods.In a word,CsCSPs are widely involved in tea plant growth,development and various stress responses.Our study provides a theoretical basis for further exploring the function of CsCSP.