基于隶属函数法的3个观赏树种抗寒性综合评价

Comprehensive Evaluation on Cold Hardiness of Three Ornamental Species by Subordinate Function

为了探讨低温胁迫对西伯利亚花楸(Sorbus sibirica)、天山花楸(Sorbus tianschanica)与欧洲荚蒾(Viburnum opulus)3个观赏树种渗透调节物质含量及抗氧化保护酶活性的变化,设-15(对照)、-20、-25、-30、-35和-40℃等6个低温处理,分别测定了这3个树种可溶性糖(SS)、可溶性蛋白(SP)、脯氨酸(Pro)、丙二醛(MDA)、相对电导率(REC)含量和超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)等保护酶活性。结果表明:随着温度的下降,西伯利亚花楸SS含量峰值出现在-40℃处理,出现较晚,抗寒性较强;在-40℃处理中西伯利亚花楸与欧洲荚蒾SP含量分别高于对照11%和27%;3个树种Pro高值均出现在-40℃处理中,其中最高为西伯利亚花楸,为0.016%,其次为欧洲荚蒾(0.013%)和天山花楸(0.010%);天山花楸-40℃处理枝条的MDA含量较高,为0.089μmol·g^(-1),说明其受低温伤害程度较重;-40℃处理西伯利亚花楸REC最低,其次为天山花楸;在-40℃处理中3个树种SOD、POD和CAT活性呈上升趋势。通过隶属函数综合分析,3个观赏树种的平均隶属度在0.410~0.500,抗寒性排序为西伯利亚花楸>天山花楸>欧洲荚蒾。

To investigate the effects of low temperature stress on osmotic regulatory substance content and antioxidant protective enzyme activity of Sorbus sibirica,Sorbus tianschanica and Viburnum opulus,the contents of soluble sugar(SS),soluble protein(SP),proline(Pro),malondialdehyde(MDA),relative electro-conduction(REC)and the activities of protective enzymes such as superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)were measured at six low temperatures-15(control),-20,-25,-30,-35 and-40.The results showed that with the temperature decreased,the peak of SS content of S.sibirica appeared at-40℃,which indicated it had a stronger cold resistance.The content of SP in S.siburica and V.opulus at-40℃were 11%and 27%higher than those in the control,respectively.The highest Pro values for three species were found at-40℃,which was 0.016%in S.siburica,0.013%in Viburnum opulus,0.010%in S.tianschanica.The content of MDA was 0.089μmol·g^(-1) in the branches treated with-40℃,which indicated that branches were seriously damaged by low temperature.The lowest REC was found in S.sibirica treated at-40℃,followed by S.tianschanica.Activities of SOD,POD and CAT of three species increased at-40℃.The average subordinate degree of three ornamental species ranges from 0.410 to 0.500 by comprehensive analysis,and the order of cold resistance is S.sibirica>S.tianschanica>V.opulus.