基于代谢组学技术探讨间歇性禁食对小鼠粪便代谢物的影响

Study on intermittent fasting changing fecal metabolism pattern of mice based on metabolomics technique

越来越多的研究表明,间歇性禁食(intermittent fasting,IF)可以改善与肥胖和糖尿病相关的风险因素,并且可以成为健康生活方式的一部分。本研究以代谢组学技术为基础,通过检测小鼠粪便代谢物探讨在正常生理情况下,间歇性禁食对小鼠粪便代谢的影响。实验方案经上海中医药大学实验动物中心福利与伦理委员会审查,符合实验动物福利与伦理相关规范。实验采用24 h隔日禁食法,对小鼠进行持续10周的间歇性禁食,并收集5周和10周时小鼠的粪便。结果表明,IF没有显著改变小鼠的体重和饮食饮水量,但可降低小鼠的空腹血糖水平;通过代谢组学分析,发现在IF第5周时就开始影响粪便代谢物水平,至第10周时影响更为显著;通过对数据的分析与鉴定,在5周和10周中分别筛选出17种和108种差异代谢物,这些差异代谢物主要富集在亚油酸代谢和胆汁酸生物合成等通路;进一步筛选发现10种代谢物在两个时间点具有相同的变化趋势,表明其可能与IF之间存在密切的相关性。本研究表明IF可显著改变小鼠的代谢水平,为其治疗相关疾病的代谢机制提供理论依据。

Increasingly researches suggested that intermittent fasting(IF)can be part of a healthy lifestyle,which can improve risk factors associated with obesity and diabetes.Based on LC-MS metabolomics technology,this study preliminarily discussed the effect of IF on the metabolism of mice under normal physiological conditions by detecting the fecal metabolites.All experiments were approved by the Animal Research Committee of Shanghai University of Traditional Chinese Medicine.Animal welfare and the animal experimental protocols were strictly consistent with related ethics regulations of Shanghai University of Traditional Chinese Medicine.The mice were subjected to intermittent fasting for 10 weeks by fasting for 24 hours on alternate days,and their feces at 5 and 10 weeks were collected.The results show that the weight,food and water intake was not significant altered in mice with IF,but fasting blood glucose level was decreased.We found that fecal metabolites change was present at 5-week,being more prominent in 10-week.Relative to the control mice,we detected 17 and 108 metabolites in two time points,respectively.These metabolites were mainly enriched in the pathways of linoleic acid metabolism and bile acid biosynthesis.We further found that 10 metabolites may be a close correlation with IF,which had the same change trend at two time points.In conclusion,the present study provides a new approach to study the metabolism mechanism in IF treatment of related diseases.