基于转录组的不同产地党参糖基转移酶鉴定及分析

Identification and analysis of glycosyltransferase in Codonopsis pilosula from different regions based on transcriptome

潞党参为山西道地药材,其药效成分党参多糖等含量显著高于其他产地党参[Codonopsis pilosula(Franch.)Nannf.]。糖基转移酶是党参主要药效成分党参多糖和党参苷Ⅰ等合成的关键酶,本研究基于不同产地党参转录组数据,对糖基转移酶基因进行GO及KEGG功能注释、保守结构域分析、进化树分析及表达模式分析,为探索潞党参道地性形成机制提供理论依据。本研究共筛选鉴定出98个糖基转移酶基因,分属于GT family 1、GT family 2、GT family 90等家族。GO功能注释发现多属于分子功能中的催化活性条目。KEGG功能注释发现党参糖基转移酶主要参与聚糖生物以及萜类和聚酮类化合物代谢。其中,GT family 1的42个糖基转移酶基因保守结构域为:[X]-W-[2X]-Q-[3X]-[LH]-[5X]-[FLTHCGWNS]-[2X]-E-[4X]-[GVP]-[4X]-P-[4X]-Q-[2X]-[NAK]。通过与拟南芥的糖基转移酶序列比对分析,党参糖基转移酶主要位于拟南芥UGT73、72、85分支中。基因表达模式分析发现党参CpUGT73AH2在潞党参植株中高表达,并可响应干旱、低温胁迫诱导。最终,本研究自党参糖基转移酶家族1中筛选得到一个参与萜类和聚酮类化合物代谢的可以响应环境胁迫的糖基转移酶基因CpUGT73AH2,为进一步研究党参糖基转移酶在潞党参道地性形成中的作用奠定了理论基础。

Lu Dangshen is the geoherb in Shanxi Province.The content of Codonopsis pilosula polysaccharides(CPP)in Lu Dangshen is more than that in other Codonopsis Radix from other regions.Glycosyltransferase is the key enzyme for the synthesis of bioactive components,such as CPP and tangshenosideⅠ.Based on the transcriptome data of C.pilosula[Codonopsis pilosula(Franch.)Nannf.]from different producing areas,this study carried out functional annotation of GO and KEGG,conservative domain analysis,phylogenetic tree analysis and expression pattern analysis of glycosyltransferase genes in C.pilosula to provides a theoretical basis for exploring the mechanism of genuineness formation in Lu Dangshen.In this study,98 glycosyltransferase genes were screened and identified,which belonged to GT family 1,GT family 2,GT family 90 and other families.By GO functional annotation,it was found that most of the glycosyltransferase genes had catalytic activity.Analysis of KEGG functional annotation showed that C.pilosula glycosyltransferase was mainly involved in glycan organism and terpenoid and polyketone metabolism.Among them,conserved domain of 42 glycosyltransferase genes in GT family 1 was[X]-W-[2X]-Q-[3X]-[LH]-[5X]-[FLTHCGWNS]-[2X]-E-[4X]-[GVP]-[4X]-P-[4X]-Q-[2X]-[NAK].Phylogenetic tree analysis based on the glycosyltransferase sequence in Arabidopsis thaliana showed that C.pilosula glycosyltransferases were mainly located in Arabidopsis thaliana UGT73,72 and 85 branches.Gene expression pattern analysis showed that expression of CpUGT73AH2 was higher in Lu Dangshen than that in Baitiaodang and could respond to drought and low temperature stress.In conclusion,a glycosyltransferase gene CpUGT73AH2,which is involved in the metabolism of terpenoids and polyketides and can respond to environmental stress,was screened from the C.pilosula glycosyltransferase family 1,which was used to further study the role of C.pilosula glycosyltransferase in Lu Dangshen.It laid a theoretical foundation for further study on the role of C.pilosula glycosyltransferase in the formation of Lu Dangshen.