茉莉酸甲酯对黄连生物碱含量积累的影响

Effect of Methyl Jasmonate on Accumulation of Alkaloids in Coptis chinensis

目的:探究茉莉酸甲酯(MeJA)对黄连中生物碱积累的影响,为进一步研究茉莉酸甲酯对黄连生物碱生物合成途径的分子调控机制提供依据。方法:用100μmol/L的茉莉酸甲酯喷施黄连幼苗叶片,于处理后0 h、24 h、48 h、72 h、96 h取样,采用高效液相色谱法对黄连幼苗中的药根碱、表小檗碱、黄连碱、盐酸巴马汀、盐酸小檗碱含量进行测定。结果:茉莉酸甲酯处理48 h后的黄连幼苗中药根碱、表小檗碱、黄连碱、盐酸巴马汀、盐酸小檗碱含量显著升高,增长率也最高,其中黄连碱对茉莉酸甲酯的响应性更好,处理48 h后的黄连幼苗中黄连碱含量为对照组的3.15倍;96 h茉莉酸甲酯处理后,黄连幼苗生物碱含量有下降趋势。结论:短时间内施加外源性茉莉酸甲酯可显著促进黄连幼苗中生物碱的积累。

Objective:To investigate the effect of methyl jasmonate(MeJA)on the accumulation of alkaloids in Coptis chinensis and to provide a basis for further research on the molecular regulation mechanism of MeJA on the biosynthesis pathway of alkaloids in C.chinensis.Methods:The leaves of C.chinensis seedlings were sprayed with 100μmol/L MeJA and sampled at 0 h,24 h,48 h,72 h,and 96 h after treatment.High-performance liquid chromatography(HPLC)was used to determine the content of jateorhizine,epiberberine,coptisine,palmatine chloride,and berberine hydrochloride in C.chinensis seedlings.Results:MeJA treatment for 48 h could significantly increase the content of jateorhizine,epiberberine,coptisine,palmatine chloride,and berberine hydrochloride in C.chinensis seedlings,with the highest growth rate.Among them,coptisine showed pronounced response to MeJA,and the content of coptisine treated for 48 h was 3.15 times that of control group.After MeJA treatment for 96 h,the content of alkaloids in C.chinensis seedlings showed a downward trend.Conclusion:The accumulation of alkaloids in C.chinensis seedlings could be significantly promoted by applying exogenous MeJA in a short time.