一测多评法测定不同寄主肉苁蓉中4种苯乙醇苷类含量

Comparison of Phenylethanoid Glycosides in Herba Cistanches with Different Hosts:Based on Quantitative Analysis of Multiple Components by Single Marker

目的:建立高压液相色谱法(HPLC)测定不同寄主植物肉苁蓉中4种苯乙醇苷类成分(松果菊苷、肉苁蓉苷A、管花苷A、毛蕊花糖苷)的含量测定方法。方法:以松果菊苷为内标,计算肉苁蓉苷A、管花苷A、毛蕊花糖苷的相对校正因子,利用一测多评法计算肉苁蓉中3种成分含量,并与外标法测定结果进行对比。结果:采用一测多评法测定6批不同寄主植物肉苁蓉中4种苯乙醇苷含量与外标法无明显差异。结论:所建立的方法准确可行,一测多评法可用于肉苁蓉的质量控制。结果表明四翅滨藜寄生肉苁蓉比梭梭寄生肉苁蓉各成分含量高。

Objective:To establish a high-performance liquid chromatography(HPLC)method for the determination of four phenylethanoid glycosides(echinacoside,cistanoside A,tubuloside A,and acteoside)in Herba Cistanches with different hosts.Methods:The relative correction factors of cistanoside A,tubuloside A,and acteoside were calculated with echinacoside as internal reference.The content of three components in Herba Cistanches was calculated by quantitative analysis of multiple components by single marker(QAMS),and the results were compared with those determined with external standard method.Results:There was no significant difference in the content of four phenylethanoid glycosides in 6 batches of Herba Cistanches with QAMS and external standard method.Conclusion:The method is accurate and feasible and can be used for the quality control of Herba Cistanches.The results showed that the content of the components in Herba Cistanches with the host of Atriplex canescens was higher than that in Herba Cistanches with the host of Haloxylon ammodendron.