不同氧分压暴露对小鼠脑和肺组织腺苷代谢通路分子及相关酶表达的影响

Effects of different oxygen partial pressure exposure on the content of molecules related to adenosine metabolism pathway and relevant enzyme expressions in the lungs and brains of mice

目的:探讨不同氧分压暴露对小鼠脑和肺组织内腺苷代谢相关分子含量及酶表达的影响。方法:36只雄性小鼠随机分为对照组、高氧性肺损伤(HILI)组和急性氧中毒(ACOT)组,每组12只。对照组仅暴露于常压空气中;HILI组动物暴露于0.25 MPa(2.5 ATA)氧分压下6 h;ACOT组动物暴露于0.40 MPa(4.0 ATA)氧分压下30 min。暴露结束后即刻,动物腹腔麻醉后取肺组织和脑组织,采用高效液相检测三磷酸腺苷(ATP)、二磷酸腺苷(ADP)、单磷酸腺苷(AMP)和腺苷(Ado)含量;采用Western blotting检测CD37、腺苷脱氨酶(ADA)和腺苷激酶(ADK)蛋白表达。结果:不同氧分压暴露可一定程度升高动物肺和脑组织内的腺苷含量,这可能与高分压氧暴露后上调腺苷合成相关酶、抑制腺苷分解相关酶的表达有关。ACOT组脑组织的变化小于肺组织。结论:不同水平的氧分压暴露会影响腺苷代谢通路,增加肺和脑内的腺苷含量,可能作为一个保护机制对抗高分压氧的不良影响。

Objective To investigate the effects of exposure to oxygen at different partial pressures on the contents of molecules related to adenosine metabolism and the expressions of relevant enzymes in the lungs and brains of mice.Methods A total of 36 male mice were randomly divided into normal control group,hyperoxia-induced lung injury(HILI)group,and acute oxygen toxicity(ACOT)group,with 12 mice in each group.In the HILI group,the mice were exposed to oxygen at 0.25 MPa(2.5 ATA)for 6 h;in the ACOT group,the mice were exposed to oxygen at 0.40 MPa(4.0 ATA)for 30 min.Immediately after hyperoxia exposure,all the mice were anesthetized intraperitoneally,and the lungs and brains were harvested for further assays.High performance liquid chromatography(HPLC)was employed for the detection of ATP,ADP,AMP,and adenosine contents;western blotting was performed for the detection of CD37,ADA,and ADK protein expressions.Results Exposure to oxygen at different partial pressures could increase the contents of adenosine in the brains and lungs to different extents,this may be ascribed to the increased expression of enzyme for the adenosine synthesis and the inhibited expression of enzyme for the adenosine catabolism.Moreover,in the ACOT group,the changes in the molecules in the lungs were more significant than those in the brains.Conclusion Exposure to oxygen at different partial pressures may affect the adenosine metabolism pathway by increasing the adenosine in the lungs and brains,which may serve as a protective response to hyperoxia exposure,thus protecting the cells against hyperoxia-induced injuries.