5-偶氮苯基-8-羟基喹啉丙烯酸酯的合成及应用

Synthesis and Application of 5-Azop henyl-8-Hydroxyquinoline Acrylate

“毛发水”酱油是一种在胱氨酸废液中添加工业盐和色素配兑成的假冒酱油。它含有4-甲基咪唑、砷、铅等有害的物质,其对人体的生殖系统、肾、血液系统、肝等均有危害,并且可以致癌。依据现有酱油的国家标准的检测手段是无法鉴别出“毛发水”酱油的。胱氨酸可以通过还原剂还原为半胱氨酸。5-偶氮苯基-8-羟基喹啉丙烯酸酯和半胱氨酸在碱性条件下可以发生1,4-亲核加成反应,形成的化合物具有大的共轭体系,能够发出强的紫外可见光或荧光,可以通过高效液相色谱紫外检测法或荧光检测法测定出半胱氨酸的含量,进而可以用该种方法检测“毛发水”酱油。

The false soy sauce was a kind of fake confirmed soy sauce which was added the waste cystine solution,pigment and industrial salt.The false soy sauce contained 4-methyl sulfonated,the arsenic,the lead and other harmful substances,which were harmful to humans,reproductive system,kidney,and the blood system and liver.What’s more it could cause cancer.The existing national detection standard of soy sauce was unable to distinguish the false soy sauce.Cystine was reduced to cysteine by the reduction.5-Azophenyl-8-Hydroxyquinoline Acrylate and cysteine could occur 1,4-nucleophilic addition reaction,the formed compounds has large conjugate system and could send strong ultraviolet light or fluorescent,so the content of cysteine could be detected by HPLC-UV or HPLC-Fluorescent,moreover,it could be used to detect the false soy sauce.