新乡地区支气管哮喘患儿发病风险与相关核转录因子等位基因间相关性探讨

Relationship between incidence of bronchial asthma and alleles of related nuclear transcription factors in children

目的探讨新乡地区支气管哮喘患儿发病风险与相关核转录因子等位基因间相关性。方法本次研究纳入河南省新乡市第二人民医院2018年1月至2020年1月收治的小儿支气管哮喘患儿260例和体检健康儿童290例,采用SSP-PCR法检测核转录因子STAT3基因单核苷酸多态性(SNP),分析两组基因型和等位基因分布差异。结果支气管哮喘组rs2293152位点CC基因型比例高于体检健康组(P<0.05);支气管哮喘组rs2293152C/G位点C等位基因比例高于体检健康组(P<0.05);C等位基因儿童发生支气管哮喘风险为G等位基因的1.57倍(OR=1.57,95%CI:1.07~2.33)。两组rs957970位点基因型和等位基因比例比较,差异无统计学意义(P>0.05)。结论本地区小儿支气管哮喘发病与STAT3基因rs2293152C/G位点有关,其中C等位基因可能为常见易感基因。

【Objective】To investigate the relationship between the incidence of bronchial asthma in children and alleles of related nuclear transcription factors in children in Xinxiang of Henan Province.【Methods】A total of 260 children with bronchial asthma and 290 healthy children in physical examination were retrospectively chosen in the period from January 2018 to January 2020 in our hospital.The SNP of STAT 3 gene was detected by SSP-PCR and the differences of genotype and allele distribution of the two groups were analyzed.【Results】The proportion of CC genotype in rs 2293152 locus of asthma group was significantly higher than that of healthy group(P<0.05).The proportion of C allele in rs 2293152 C/G locus of asthma group was significantly higher than that of healthy group(P<0.05).The risk of asthma in children with C allele was 1.57 times higher than that in G allele(OR=1.57,95%CI:1.07-2.33).There was no significant difference in genotype and allele proportion in rs 957970 between the two groups(P>0.05).【Conclusion】The rs 2293152 C/G locus of STAT 3 gene is associated with the onset of bronchial asthma in children in this region and C allele may be the common susceptible gene.