电感耦合等离子体质谱法测定中药材中15种金属元素的含量

Determining the Contents of 15 Metallic Elements in Traditional Chinese Medicinal Materials by ICP-MS

目的:建立电感耦合等离子体质谱法(ICP-MS)测定中药材中锂(Li)、铍(Be)、钴(Co)、镍(Ni)、砷(As)、硒(Se)、锶(Sr)、锰(Mn)、镉(Cd)、锌(Zn)、铝(Al)、铊(Tl)、铅(Pb)、铋(Bi)、铜(Cu)15种金属元素的含量。方法:取中药材样品,60℃烘干3 h,粉碎成粗粉。精密称取0.2 g,置消解罐内进行消解,最后用水定容至25 mL。建立ICP-MS方法测定样品中15种金属元素的含量,外标法定量。ICP-MS射频功率为1150 W,雾化器氩气流速为1.20 L·min^(-1),等离子体氩气流速为15 L·min^(-1),采样深度为8 mm,反应模式为标准模式和碰撞模式。结果:在最佳仪器条件下,15种金属元素在0~100μg·L^(-1)范围内线性关系良好,相关系数均大于0.999,平均回收率为85.3%~114.7%,精密度的相对标准偏差(RSD)为0.68%~5.65%,检出限均低于0.047μg·L^(-1),符合分析技术要求。结论:该方法快速、简便、重复性好、准确度高,可用于中药材中15种金属元素含量的测定。

Objective:To establish a inductively coupled plasma mass spectrometry(ICP-MS)method for simultaneous determination of 15 metallic elements,Lithium(Li),Beryllium(Be),Cobalt(Co),Nickel(Ni),Arsenicum(As),Selenium(Se),Strontium(Sr),Manganese(Mn),Cadmium(Cd),Zinc(Zn),Aluminum(Al),Thallium(Tl),Plumbum(Pb),Bismuth(Bi),Cuprum(Cu),in traditional Chinese medicinal materials.Methods:Samples of traditional Chinese medicinal materials were dried at 60℃for 3 h and crushed for coarse powder.Accurately weighed 0.2 g coarse powder of the sample,and placed into the digestion tank for digestion.Finally,the metered volume should be fixed to 25 mL by water.The ICP-MS method was established to determine the contents of 15 metallic elements,and external standard method was used for quantification.The instrument parameters of ICP-MS were as follows:radio frequency(RF)power at 1150 W,atomizer flow rate of argon gas at 1.20 L·min^(-1),plasma flow rate of argon gas at 15 L·min^(-1),sampling depth at 8 mm.The reaction modes were standard mode and collision mode.Results:Under the optimal instrument conditions,the linearity was good for 15 metallic elements in the concentration range of 0-100μg·L^(-1),and the correlative coefficients was higher than 0.999.The recovery rate ranged from 85.3%to 114.7%.The RSD for precision ranged from 0.68%to 5.65%.The detection limits were all lower than 0.047μg·L^(-1).The verified data meet the requirements of the analytical method.Conclusion:The method is rapid,simple,reproducible and accurate,and can be used for the content determination of 15 metallic elements in traditional Chinese medicinal materials.