替米考星原粉和可溶性粉在单次给药肉鸡的药代动力学研究

Pharmacokinetic study of tilmicosin API and tilmicosin soluble powder with a single dose in broiler chickens

该试验评估了替米考星原粉和37.5%替米考星可溶性粉在肉鸡体内的药代动力学行为。将8只肉鸡随机分组,以30 mg/kg灌服替米考星原粉和37.5%替米考星可溶性粉,用UPLC-MS/MS检测血浆中替米考星的质量浓度,采用Winnonlin软件计算其药动学参数。结果表明:37.5%替米考星可溶性粉的峰浓度显著高于原粉(10.3±2.0μg/mL vs 1.4±0.4μg/mL),由此结果可推测肉鸡吸收可溶性粉的速度快于原粉。替米考星可溶性粉的AUC_(all)和AUC_(inf)均大于原粉的AUC_(all)和AUC_(inf)(31.2±9.2μg·h·mL^(-1)vs 11.7±3.2μg·h·mL^(-1);36.7±11.7μg·h·mL^(-1)vs 15.8±3.4μg·h·mL^(-1)),表明替米考星可溶性粉到达全身血液循环的量多于原粉。

The pharmacokinetic study of tilmicosin active pharmaceutical ingredients(API)and 37.5%tilmicosin soluble powder in broiler chickens were conducted.Eight healthy broiler chickens were randomly divided into two groups and they were administered at a dose of 30 mg/kg of tilmicosin API and 37.5%tilmicosin soluble powder,respectively.The plasma concentrations of tilmicosin were determined by UPLC-MS/MS and pharmacokinetic parameters were calculated by Winnonlin software.The results showed that the peak concentrations of tilmicosin soluble powder were significantly higher than that of tilmicosin API(10.3±2.0μg/mL vs 1.4±0.4μg/mL),indicating that the absorption rate of tilmicosin soluble powder was greater than that of tilmicosin API.We also found that AUC_(all) and AUCinf of the tilmicosin solublepowderwere greater than that of tilmicosin API,demonstrating that the amount of tilmicosin soluble powder reaching the whole blood circulation in broiler chickens was greater than that of the tilmicosin API.