VSTM2A、MICA基因多态性与非小细胞肺癌发病风险及患者临床病理特征的关系

Relationship between VSTM2A,MICA gene polymorphisms and the risk of non-small cell lung cancer and the clinicopathological characteristics of patients

目的探讨V-组跨膜结构域包含物2A(VSTM2A)、主要组织相容性复合体Ⅰ类相关基因A(MICA)的基因多态性与非小细胞肺癌(NSCLC)发病风险及患者临床病理特征的关系。方法选取2019年1月至2020年1月陕西中医药大学附属医院收治的90例NSCLC患者为肺癌组,另选取同期90例健康志愿者为对照组,检测两组VSTM2A-rs7457728和MICA-rs2516448位点的基因多态性,比较两组患者上述位点的基因型及等位基因分布情况,采用Logistic回归分析上述位点基因型对NSCLC发病的影响,并分析上述位点基因多态性与NSCLC患者临床病理特征的关系。结果肺癌组VSTM2A-rs7457728位点C等位基因、CC基因型频率明显高于对照组,差异有统计学意义(P<0.05)。对照组MICA-rs2516448位点T等位基因、TT基因型频率明显低于肺癌组,差异有统计学意义(P<0.05)。Logistic回归分析发现,携带VSTM2A-rs7457728位点CC基因型、MICA-rs2516448位点TT基因型是NSCLC发病的危险因素(P<0.05)。腺癌患者MICA-rs2516448位点TT基因型频率明显高于鳞癌患者,差异有统计学意义(P<0.05)。结论VSTM2A-rs7457728位点C等位基因突变和MICA-rs2516448位点T等位基因突变可增加NSCLC的发病风险,并且MICA-rs2516448位点TT基因型与NSCLC的病理类型有关。

Objective To investigate the relationship between genetic polymorphisms of V-group transmembrane domain inclusion 2A(VSTM2A)and major histocompatibility complex classⅠassociated gene A(MICA)and the risk of non-small cell lung cancer(NSCLC)and the clinicopathological characteristics of patients.Methods A total of 90 NSCLC patients admitted to the Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine from January 2019 to January 2020 were selected as the lung cancer group,and 90 healthy volunteers during the same period were selected as the control group.The gene polymorphisms of VSTM2A-rs7457728 and MICA-rs2516448 locus in the two groups were detected.Compared the genotype and allele distribution of the above locus in the two groups of patients.Logistic regression was used to analyze the influence of the above locus genotypes on the incidence of NSCLC.The relationship between the gene polymorphisms of the above locus and the clinicopathological characteristics of NSCLC patients was also analyzed.Results The frequency of C allele and CC genotype of VSTM2A-rs7457728 locus in the lung cancer group was significantly higher than that in the control group,and the differences were statistically significant(P<0.05).The frequency of T allele and TT genotype of MICA-rs2516448 locus in the control group was significantly lower than that in the lung cancer group,and the differences were statistically significant(P<0.05).Logistic regression analysis showed that carrying VSTM2A-rs7457728 locus CC genotype and MICA-rs2516448 locus TT genotype were risk factors for NSCLC(P<0.05).The TT genotype frequency of MICA-RS2516448 locus in adenocarcinoma patients was significantly higher than that in squamous cell carcinoma patients,the difference was statistically significant(P<0.05).Conclusion VSTM2A-rs7457728 locus C allele mutation and MICA-rs2516448 locus T allele mutation can increase the risk of NSCLC,and MICA-rs2516448 locus TT genotype was associated with the pathological type of NSCLC.