马铃薯淀粉的场流分离表征过程回收率研究

Recovery of potato strach during separation and characterization process using field-flow fractionation

采用场流分离技术探究了马铃薯(Solanum tuberosum L.)淀粉分离表征过程中样品间以及样品与超滤膜的交联,评价了载液离子强度、样品进样量、交叉流流速和聚集时间对马铃薯淀粉回收率的影响,并对结果重复性进行了考察.结果表明:在载液(含5 mmol/L NaNO_(3),3 mmol/L NaN_(3))pH 7.00,样品进样量15μg,交叉流流速1.0 mL/min,聚集时间2.5 min的条件下,马铃薯淀粉的回收率为(97.30±0.02)%,回转半径相对标准偏差为1.18%,分子摩尔质量的相对标准偏差为5.67%.

Potato starch was separated and characterized by field-flow fractionation.The interactions between the sample and the surface of ultrafiltration membrane in AF4 were investigated.The effects of ionic strength of carrier liquid,injection mass,cross-flow rate,and focusing time on the sample recovery were evaluated.The repeatability of the results was examined.The highest recovery of potato starch(97.30±0.02)% was obtained with a carrier liquid containing 5 mmol/L NaNO_(3) and 3 mmol/L NaN_(3),pH 7.00;injection mass,15μg;cross-flow rate,1.0 mL/min;focusing time,2.5 min.The method developed in this study showed good repeatability in terms of radius of gyration and molar mass with their relative standard deviations of 1.18% and 5.67%,respectively.