病毒感染性腹泻对儿童人群肠道菌群及IL-6、TNF-α、hs-CRP、IFN-γ水平的影响

Effect of virus-infected diarrhea on intestinal flora and levels of IL-6,TNF-α,hs-CRP and IFN-γin children

目的 探讨病毒感染性腹泻对儿童人群肠道菌群及白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、超敏C反应蛋白(hs-CRP)、干扰素-γ(IFN-γ)水平的影响。方法 选取2019年1月至2021年1月在就诊的感染性腹泻患儿165例,其中病毒感染性腹泻98例,细菌性腹泻67例,同时选取非感染性腹泻患儿100例作为对照,分析比较各患儿肠道菌群、血清IL-6、TNF-α、hs-CRP、IFN-γ水平差异。结果 细菌感染性和病毒感染性腹泻患儿双歧杆菌属、乳酸杆菌属、梭菌属和梭杆菌属明显低于非感染性腹泻患儿(P<0.05),其中病毒感染性腹泻患儿双歧杆菌属、乳酸杆菌属、梭菌属和梭杆菌属分别为(7.02±0.90)logCFU/g、(7.41±0.84)logCFU/g、(7.02±0.91)logCFU/g和(6.11±0.89)logCFU/g,明显高于细菌感染性腹泻(P<0.05);细菌感染性和病毒感染性腹泻患儿IL-6、TNF-α、hs-CRP和IFN-γ明显高于非感染性腹泻患儿(P<0.05),其中病毒感染性腹泻患儿IL-6、TNF-α、hs-CRP和IFN-γ分别为(13.39±4.10)pg/ml、(178.29±56.65)ng/L、(9.83±2.10)mg/L和(4.09±1.03)pg/ml,明显低于细菌感染性腹泻(P<0.05);杯状病毒、轮状病毒及其他病毒感染性腹泻患儿肠道菌群比较差异无统计学意义(P>0.05);轮状病毒感染性腹泻患儿血清IFN-γ为(4.49±0.91)pg/ml,明显高于杯状病毒和其他病毒感染性腹泻患儿(P<0.05);杯状病毒、轮状病毒和其他病毒感染性腹泻患儿血清IL-6、TNF-α和hs-CRP比较差异无统计学意义(P>0.05)。结论 相较于细菌感染性腹泻,病毒感染性腹泻患儿肠道菌群、血清IL-6、TNF-α、hs-CRP、IFN-γ异常程度轻,其中IFN-γ在不同病原病毒感染性腹泻中有所差异。

Objective To investigate the effects of virus-infected diarrhea on intestinal flora and the levels of interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),hypersensitive C-reactive protein(hs-CRP)and interferon-γ(IFN-γ)in children.Methods A total of 165children with infectious diarrhea admitted to our hospital from January 2019to January 2021were selected,including 98cases of viral diarrhea and 67cases of bacterial diarrhea,and 100cases of non-infectious diarrhea were selected as controls,the differences in intestinal flora,serum IL-6,TNF-α,hs-CRP and IFN-γlevels were analyzed and compared among the children.Results The Bifidobacterium,Lactobacillus,Clostridium and Fusobacterium in children with bacterial and viral diarrhea were significantly lower than those in children with non-infectious diarrhea(P0.05);The serum IFN-γin children with rotavirus infectionwas(4.49±0.91)pg/ml,which was significantly higher than that in children with calicivirus and other viruses infection(P0.05).Conclusion Compare with bacterial diarrhea,the abnormal degree of intestinal flora,serum,IL-6,TNF-α,hs-CRP and IFN-γin the children with viral diarrhea are less,and IFN-γis different in the diarrhea with different pathogenic viruses.