蚊浓核病毒亚致死剂量感染对白纹伊蚊溴氰菊酯抗药性的影响研究

Effect of sublethal dose of Mosquito densovirus infection on deltamethrin resistance of Aedes albopictus

目的研究白纹伊蚊浓核病毒-7(AalDV-7)亚致死剂量感染对白纹伊蚊溴氰菊酯抗药性的影响。方法对实验室白纹伊蚊敏感品系施加溴氰菊酯进行筛选,建立抗性品系。利用亚致死剂量(108 geq/ml)的AalDV-7感染白纹伊蚊幼虫,通过抗药性生物测定法检测感染组和未感染组幼蚊的半数致死浓度(LC_(50))和成蚊死亡率,使用SPSS 19.0软件通过t检验与χ^(2)检验分别比较幼蚊LC_(50)与成蚊死亡率。结果经15代筛选成功建立了实验室白纹伊蚊溴氰菊酯抗性品系。亚致死剂量AalDV-7感染后,幼蚊LC_(50)由0.0300 mg/L降低至0.0185 mg/L(t=12.521,P<0.001),降为中等抗性,成蚊抗药性生物测定法死亡率由65.7%提高到78.1%(χ^(2)=5.079,P=0.015),但抗性水平未变。结论亚致死剂量AalDV-7感染可降低白纹伊蚊幼虫溴氰菊酯抗药性,在一定程度上起到了增效剂的作用,结果为解决蚊媒抗药性提供了新的理论依据与策略。

Objective To study the effect of sublethal dose of Aedes albopictus densovirus-7(AalDV-7)infection on the deltamethrin resistance of Ae.albopictus.Methods The laboratory resistant strain of Ae.albopictus was established by applying deltamethrin insecticide to the laboratory susceptible strain.Ae.albopictus larvae were infected with a sublethal dose of AalDV-7(108 geq/ml).The half-lethal concentration(LC_(50))for larvae and the biological mortality of adult mosquitos in the infected and uninfected groups were determined using the resistance bioassay,and were compared by the t test and χ^(2) test using the SPSS 19.0 software,respectively.Results The laboratory strain of Ae.albopictus resistant to deltamethrin was successfully established after 15 consecutive generations of screening.After sublethal dose ofAalDV-7 infection,the LC_(50) for larvae decreased from 0.0300 mg/L to 0.0185 mg/L(moderate resistance)(t=12.521,P<0.001),and the biological mortality of adult mosquitoes increased from 65.7% to 78.1%(χ^(2)=5.079,P=0.015),with the resistance level unchanged.Conclusion Sublethal dose of AalDV-7 infection reduces the deltamethrin resistance of Ae.albopictus larvae and acts as a synergist to some extent,providing a new theoretical basis and strategy for solving mosquito resistance.