摘要
采用HP20、硅胶、ODS以及半制备液相等多种色谱手段对连翘果实75%乙醇提取浸膏乙酸乙酯萃取部位的化学成分进行分离纯化,并根据理化性质和波谱数据鉴定其结构,得到1个新的苯乙醇苷类化合物,命名为连翘炎苷E(1)。采用MTT法测定化合物对MCF-7、A-375、PC-3和HepG2细胞系的抑制活性,化合物1在40μmol·L^(-1)浓度下对MCF-7和A-375细胞系的抑制率分别为39.85%、43.38%,在100μmol·L^(-1)浓度下对PC-3和HepG2细胞系没有抑制活性。
One new phenylethanoid glycoside was isolated from the ethyl acetate fraction of the 75%EtOH extract of Forsythiae Fructus by various column chromatographies(HP20,silica gel,ODS)and preparative HPLC.Its structure was identified as forsythiayanoside E(1)by physicochemical properties and extensive spectroscopic analysis(HR-ESI-MS,1 D and 2 D NMR).Compound 1 was evaluated for cytotoxic activities by MTT assay and showed weak cytotoxic activity against MCF-7 and A-375 cell lines with inhibition rates of 39.85%and 43.38%at 40μmol·L^(-1),and no cytotoxic activity against PC-3 and HepG2 cell lines at 100μmol·L^(-1).
作者
阎新佳
聂承冬
江园园
李畅
温静
沙马里牛
沙冬梅
刘圆
YAN Xin-jia;NIE Cheng-dong;JIANG Yuan-yuan;LI Chang;WEN Jing;SHA Ma-li-niu;SHA Dong-mei;LIU Yuan(Institute of Qinghai-Tibetan Plateau,Southwest Minzu University,Chengdu 610041,China;Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory of National Ethnic Affairs Commission of the People′s Republic of China,Chengdu 610225,China;Sichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology Engineering Laboratory,Chengdu 610225,China;College of Pharmacy,Harbin University of Commerce,Harbin,150076,China;College of Pharmacy,Harbin Medical University,Harbin,150081,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2022年第13期3526-3529,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金青年基金项目(81803696)
黑龙江省自然科学基金优秀青年项目(YQ2020H023)
西南民族大学科研启动金项目(RQD2022050)。
关键词
连翘
苯乙醇苷类
连翘炎苷E
结构鉴定
抗肿瘤活性
Forsythiae Fructus
phenylethanoid glycoside
forsythiayanoside E
structural identification
anti-tumor activity
