基于UPLC特征图谱与一测多评法的人参药材质量评价研究

Quality evaluation of Ginseng Radix et Rhizoma based on UPLC characteristic chromatogram and quantitative analysis of multi-components by single marker(QAMS)

基于UPLC特征图谱、一测多评法同时测定人参药材中7种人参皂苷成分的含量,结合特征图谱进行主成分分析,评价人参药材质量。使用Acquity UPLC BEH C_(18)色谱柱(2.1 mm×100 mm,1.7μm),以乙腈-水作为流动相梯度洗脱,流速0.3 mL·min^(-1),柱温30℃,检测波长203 nm,进样量2μL。对3个产地19批人参药材进样分析,通过《中药色谱指纹图谱相似度评价系统》(2012版)建立了人参药材UPLC特征图谱,共确定了13个特征峰,指认出其中7种人参皂苷成分,采用SPSS 26.0软件对特征峰峰面积进行主成分分析。以人参皂苷Rb_(1)为参照峰S,人参皂苷Rb_(1)相对其他人参皂苷成分相对校正因子耐用性良好,建立了人参药材7种皂苷成分一测多评测定方法,含量测定结果与外标法相比无显著差异。主成分分析结果与7种人参皂苷总含量测定结果均表明,编号S19、S18、S1、S2的4批人参药材质量较佳。该研究建立的特征图谱及7种人参皂苷一测多评方法便捷、准确,大幅缩短了分析时间,提高了分析效率,为人参药材质量整体评价提供了依据。

Based on UPLC characteristic chromatogram and quantitative analysis of multi-components by single marker(QAMS),the content of seven types of ginsenosides in Ginseng Radix et Rhizoma was simultaneously determined,and the quality of Ginseng Radix et Rhizoma was evaluated by the principal component analysis(PCA).The chromatographic separation was performed on the Acquity UPLC BEH C_(18)column(2.1 mm×100 mm,1.7μm)with the mobile phase of acetonitrile-water for gradient elution at the flow rate of 0.3 mL·min^(-1),the column temperature of 30℃,the detection wavelength of 203 nm,and the injection volume of 2μL.The UPLC chromatogram was established with 19 batches of Ginseng Radix et Rhizoma samples from three producing areas by Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(version 2012).Thirteen characteristic peaks were determined and seven components were identified.SPSS 26.0 was used to conduct PCA on the characteristic peak areas.With the peak of ginsenoside Rb_(1) as reference peak S,ginsenoside Rb_(1) showed good durability of relative correction factor as compared with other ginsenosides.The QAMS method for the determination of seven ginsenosides in Ginseng Radix et Rhizoma was established.There was no significant difference in results between the QAMS method and the external standard method.As revealed by the results of PCA and the determination of the total content of seven ginsenosides,the four batches of Ginseng Radix et Rhizoma numbered S19,S18,S1,and S2 were of superior quality.The characteristic chromatogram and QAMS method for the determination of seven ginsenosides in this study were convenient and accurate,which greatly shortened the analysis time and improved the analysis efficiency.The findings of this study are expected to provide a basis for the overall quality evaluation of Ginseng Radix et Rhizoma.