环介导等温扩增技术在感染性疾病病原诊断中的应用研究进展

Application of loop mediated isothermal amplification in etiological diagnosis of infectious diseases

感染仍是婴幼儿致病的重要原因。因年龄小、免疫功能不健全,儿童感染具有"急"、"重"、"致病原复杂"等特征,往往会造成严重后果,其快速、准确诊断尤为重要。然而,现有病原检测手段仍较为局限,存在检测效率低、周期长、受制于实验条件及人员等缺点,较难普及。以环介导等温扩增技术为基础的病原学检测手段效率高、反应成本低廉、操作简单,在儿童病原的快速、准确、低成本检测中具有巨大应用潜力,特别适合在基层医疗单位进行推广。环介导等温扩增技术与纸基微流控芯片、智能自动化系统、数字化系统等技术结合后,无疑将为儿童临床病原学诊断带来重大革新。

Infectious diseases are still the most important diseases in infants and young children.Due to young age and imperfect immune function,infectious diseases in children are characterized by"urgent","serious"and"complex pathogens",which often lead to serious complications.Rapid and accurate diagnosis is particularly important for children.However,the existing pathogen detection methods are inefficiency,subject to experimental conditions and person,and impopularized in hospitals.The pathogen detection method based on loop mediated isothermal amplification presents high specificity,low-cost and simple operation and is suitable for promotion in primary medical units,which has great application value in the detection of pathogens of children.In addition,the combination of loop mediated isothermal with paper-based micro-gene chip,intelligent automation and digital system will make progress to clinical etiological diagnosis.