上海地区成人网织红细胞相关参数参考区间的建立

Establishment of the reference intervals for related parameters of adult reticulocytes in Shanghai

目的建立上海地区成人网织红细胞(RET)相关参数的参考区间。方法选取2080名体检健康者,检测RET相关参数[网织红细胞绝对值(RET#)、网织红细胞百分比(RET%)、高荧光强度网织红细胞百分比(HFR%)、中荧光强度网织红细胞百分比(MFR%)、低荧光强度网织红细胞百分比(LFR%)、未成熟网织红细胞百分比(IRF%)、网织红细胞血红蛋白含量(RET-He)、网织红细胞生成指数(RPI)]。按性别和年龄(18~40岁、41~60岁和61~80岁)分别分组进行比较,建立参考区间。另收集40份健康体检者血常规检测样本,用于参考区间验证。结果正态性检验结果显示,RET相关参数均呈偏态分布(P<0.05)。RET%、RET#、RPI各年龄组之间差异均无统计学意义(P>0.05)。41~60岁组、61~80岁组IRF%、LFR%、MFR%、HFR%、RET-He与18~40岁组比较差异均有统计学意义(P<0.05),而41~60岁组与61~80岁组之间差异均无统计学意义(P>0.05)。合并年龄、性别各组后采用百分位数法建立参考区间:RET%、RET#、RPI男性分别为0.92%~2.15%、(46.30~107.30)×10^(9)/L、0.9~2.4,女性分别为0.87%~2.27%、(37.96~101.90)×10^(9)/L、0.6~1.8;IRF%、LFR%、MFR%、HFR%18~40岁男性分别为4.1%~16.1%、83.9%~96.0%、3.6%~12.1%、0.2%~5.0%,女性分别为3.9%~17.9%、82.1%~96.1%、3.6%~12.5%、0.1%~5.5%,41~80岁(不分性别)分别为4.4%~18.0%、82.0%~95.6%、4.0%~12.8%、0.2%~5.1%;RET-He 18~40岁男性为30.3~35.5 pg、女性为27.6~34.6 pg,41~80岁男性为30.4~35.7 pg、女性为27.8~34.6 pg。40份验证样本检测结果均在建立的参考区间内。结论初步建立了上海地区成人RET相关参数的参考区间,可提高RET检测的临床应用价值。

Objective To establish the reference intervals for adult reticulocyte(RET)-related parameters in Shanghai.Methods The RET-related parameters[the absolute value of reticulocyte(RET#),the percentage of reticulocyte(RET%),high fluorescence intensity reticulocyte percentage(HFR%),medium fluorescence intensity reticulocyte percentage(MFR%),low fluorescence intensity reticulocyte percentage(LFR%),immature reticulocyte fraction percentage(IRF%),reticulocyte hemoglobin(RET-He)and reticulocyte production index(RPI)]of 2080 healthy adults were determined.According to sex,the research subjects were classified into male and female groups,and according to ages,they were classified into 18-40-year-old,41-60-year-old and 61-80-year-old groups.Another 40 samples for blood routine test were collected for the verification of reference intervals.Results The results of normality test showed that the RET-related parameters were skewed distribution(P<0.05).There was no statistical significance in RET%,RET#and RPI among different age groups(P>0.05).There was statistical significance in IRF%,LFR%,MFR%,HFR%and RET-He between 41-60-year-old group,61-80-year-old group and 18-40-year-old group(P<0.05),while there was no statistical significance between 41-60-year-old and 61-80-year-old groups(P>0.05).The reference intervals were established by percentile method after combining age and sex groups.The reference intervals of RET%,RET#and RPI in males were 0.92%-2.15%,(46.30-107.30)×10^(9)/L,0.9-2.4,and those in females were 0.87%-2.27%,(37.96-101.90)×10^(9)/L,0.6-1.8,respectively.The reference intervals of IRF%,LFR%,MFR%and HFR%in 18-40-year-old males were 4.1%-16.1%,83.9%-96.0%,3.6%-12.1%,0.2%-5.0%,those in 18-40-year-old females were 3.9%-17.9%,82.1%-96.1%,3.6%-12.5%,0.1%-5.5%,and those in 41-80-year-old males and females were 4.4%-18.0%,82.0%-95.6%,4.0%-12.8%,0.2%-5.1%,respectively.The reference interval of RET-He in 18-40-year-old males was 30.3-35.5 pg,that in 18-40-year-old females was 27.6-34.6 pg,that in 41-80-year-old males was 30.4-35.7 pg,and that in 41-80-year-old females was 27.8-34.6 pg.The results of 40 verification samples were all in the reference intervals.Conclusions The reference intervals of RET-related parameters for adults in Shanghai have been established preliminarily,which can improve the clinical application roles of RET-related parameters.