十二个茶树品种对茶棍蓟马、茶小绿叶蝉抗性表现及抗性成分初步鉴定

Resistance of 12 tea cultivars to Dendrothrips minowai Priesner and Empoasca Onukii Matsuda and a preliminary identification of resistant components

为明确贵州主栽茶树品种对茶棍蓟马(Dendrothrips minowai Priesner)、茶小绿叶蝉(Empoasca onukii Matsuda)的抗性表现及相关抗性生化因子,利用为期1年的田间调查明确12个茶树品种抗性表现,采用高效液相色谱法(HPLC)检测茶树中的咖啡碱、氨基酸组分、儿茶素组分等27种生化成分,用相关性分析判断生化含量与品种抗性间的关系。结果表明:12个茶树品种中,乌牛早、金观音和中茶108为茶棍蓟马高感品种,苔选0310和黔辐4号为茶棍蓟马高抗品种;福鼎大白茶为茶小绿叶蝉高感品种,黔湄601为茶小绿叶蝉高抗品种。咖啡碱含量与茶棍蓟马发生量呈显著负相关,大部分氨基酸含量与茶棍蓟马及茶小绿叶蝉发生量呈正相关,其中精氨酸含量与茶小绿叶蝉发生量呈显著正相关。除表没食子儿茶素外的5个儿茶素单体含量与茶棍蓟马及茶小绿叶蝉发生量呈负相关,其中儿茶素含量与茶小绿叶蝉发生量呈显著负相关。

This article aims to ascertain the resistance of 12 tea cultivars to Dendrothrips minowai Priesner and Empoasca Onukii Matsuda,and to clarify the related resistance biochemical components.Through a one-year field survey,the resistance performance of 12 tea cultivars to D.minowai and E.onukii were confirmed.HPLC was used to analyze caffeine,amino acids,catechin components and the resistance biochemical components were also defined through correlation analysis.Results showed,Wuniuzao,Jinguanyin,Zhongcha 108 were more susceptible to D.minowai.In contrast,Taixuan 0310 and Qianfu 4 possessed higher resistance.Fuding Dabaicha was more susceptible to E.onukii.In contrast,Qianmei 601 possessed higher resistance.The occurrence quantity of D.minowai was negatively significant correlated with caffeine content.Most amino acids contribute to the susceptibility of E.onukii.Arginine was positively significant correlated to the occurrence quantity of E.onukii.Except epigallocatechin,the other 5 catechin monomers contribute to the resistance of E.onukii.The content of(+)-catechin was negatively significant correlated to the occurrence quantity of E.onukii.