脊柱结核患者骨密度与骨髓间充质干细胞迁徙研究

Bone mineral density and migration of bone marrow mesenchymal stem cells in patients with spinal tuberculosis

目的探究脊柱结核患者骨密度和骨髓间充质干细胞(BMSCs)迁徙能力的变化及相关性。方法选取2020年6月1日—2021年12月1日某院住院的脊柱结核行脊柱内固定患者(结核组,72例)和胸腰椎骨折或椎管狭窄行脊柱内固定的患者(对照组,76例),测定患者骨密度,结核组和对照组各采集6例患者手术时取的钉道血提取BMSCs,将提取的BMSCs进行单纯培养和卡介苗(BCG)共培养(即在BMSCs中加入BCG进行共培养),采用划痕试验测定各组第8小时、第16小时、第24小时BMSCs迁徙率,比较各组BMSCs迁徙能力的差异,并对BMSCs标本来源患者骨密度和BMSCs迁徙率作相关性分析。结果结核组患者的骨密度[(0.84±0.19)g/cm^(2)]低于对照组[(0.95±0.16)g/cm^(2)],差异有统计学意义(P<0.05);结核组患者BMSCs迁徙率低于对照组(P<0.05);加入BCG共培养后,结核组患者BMSCs迁徙能力依旧明显降低(P<0.05)。在第8小时和第16小时BMSCs迁徙率与其标本来源患者的椎体骨密度相关系数(r值)分别为0.80、0.67。结论较胸腰椎骨折或椎管狭窄者患者,脊柱结核患者骨密度明显降低,脊柱结核患者BMSCs迁徙能力减弱;BCG对BMSCs的迁徙能力无明显影响,骨密度与BMSCs迁徙率呈正相关。

Objective To explore the changes and correlation between bone mineral density and migration ability of bone marrow mesenchymal stem cells(BMSCs) in patients with spinal tuberculosis. Methods From June 1, 2020 to December 1, 2021, 72 patients with spinal tuberculosis underwent spinal internal fixation(tuberculosis group) and 76 patients with thoracolumbar fracture or spinal stenosis underwent spinal internal fixation(control group) in a hospital were selected, bone mineral density of patients was determined, BMSCs were extracted from blood in screw path of 6 patients in tuberculosis group and control group respectively, the extracted BMSCs were cultured simply and co-cultured with BCG(BCG was added to BMSCs for co-culture), the migration rates of BMSCsat the 8 th, 16 th and 24 th hours in each group was measured by scratch test, difference of migration ability of BMSCs in each group was compared, correlation between bone mineral density and migration rate of BMSCs was analyzed. Results Bone mineral density([0.84±0.19] g/cm^(2)) in tuberculosis group was lower than that in control group([0.95±0.16] g/cm^(2)), difference was significant(P<0.05);migration rate of BMSCs in tuberculosis group was lower than that in control group(P<0.05);after co-culture with BCG, the migration ability of BMSCs in tuberculosis group still significantly decreased(P<0.05). The correlation coefficient(r value) between migration rate of BMSCs and vertebral bone mineral density at the 8 th and 16 th hours were 0.80 and 0.67 respectively. Conclusion Compared with patients with thoracolumbar fracture or spinal stenosis, bone mineral density of patients with spinal tuberculosis is significantly lower, migration ability of BMSCs of patients with spinal tuberculosis is weakened;BCG has no significant effect on the migration ability of BMSCs, and bone mineral density is positively correlated with migration rate of BMSCs.