摘要
Silver nanoparticles(Ag NPs),relative to existing antibacterial agents,are more effective,less toxic and more economical,and have shown enormous potential for the nanomedicine application.In this work,we report a‘green’method for the rapid and efficient synthesis of Ag NPs using Ginkgo biloba extracts as reducing agent and capping agent.The properties of Ag NPs against fungi and bacteria were investigated.The results showed that the Ginkgo biloba extracts are crucial for the preparation of uniform and monodispersed Ag NPs.The prepared Ag NPs exhibited remarkable antibacterial activities.The minimum inhibitory concentrations of Ag NPs for Escherichia coli and Pseudomonas aeruginosa were 0.044 and 0.088μg·mL−1,respectively.Moreover,Ag NPs exhibited excellent bactericidal performance against MDR-Pseudomonas aeruginosa.It was found that the effect of the antibacterial activity of Ag NPs on Escherichia coli and Staphylococcus aureus was tightly related to the reactive oxygen species accumulation.This research provides guidelines for the efficient green synthesis of Ag NPs and its antibacterial applications.
基金
supports by the National Natural Science Foundation of China(Grant Nos.51871196 and 81960322)
the Yunnan Fundamental Research Projects(Grant No.202001BB050046)
the Major Science and Technology Project of Precious Metal Materials Genetic Engineering in Yunnan Province(Grant No.202002AB080001)
the joint fund of Yunman University and Science&Technology Depatment of Y unnan Provinee(Grant No.2019FY003013)
the Medical Reserve Personnel Training Program of Yunman Prorincial Health Comision(Grant No.H-2018097).
