头穴艾灸联合跑台训练对血管性痴呆大鼠学习记忆和氧化应激的效果

Effects of scalp moxibustion combined with treadmill training on learning and memory,and oxidative stress in rats with vascular dementia

目的 观察头穴艾灸联合跑台训练对血管性痴呆(VaD)模型大鼠学习记忆能力和氧化应激损伤的影响。方法 健康雄性Sprague-Dawley大鼠40只,随机分为假手术组、模型组、针康组和灸康组,每组10只。后三组采用双侧颈动脉结扎法制备VaD模型大鼠。造模后6周采用Morris水迷宫测试从造模大鼠中筛选出明显认知障碍大鼠每组8只,假手术组随机挑选8只。针康组予头穴丛刺结合跑台训练,灸康组予头穴艾灸结合跑台训练,共4周。干预后行Morris水迷宫测试,检测海马组织丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)和谷胱甘肽过氧化物还原酶(GSH-Px)水平。结果 与假手术组相比,模型组大鼠逃避潜伏期显著延长,穿越平台次数显著减少,目标象限停留时间显著缩短(P <0.001),SOD、GSH、GSH-Px水平显著下降,MDA水平显著增加(P <0.001);针康组和灸康组实验第3天起逃避潜伏期较模型组显著缩短(P <0.001),穿越平台次数显著增多,目标象限停留时间显著延长(P <0.001);SOD、GSH、GSH-Px水平显著增高,MDA水平显著下降(P <0.001),且灸康组显著优于针康组(P <0.001)。结论 与针康法相比,头穴艾灸结合跑台训练可进一步降低VaD大鼠海马组织氧化应激水平,改善学习与记忆功能。

Objective To observe the effects of scalp moxibustion combined with treadmill training on the learning and memory function,and oxidative stress in rats with vascular dementia(VaD).Methods A total of 40 male Sprague-Dawley rats were randomly divided into sham group(n=10),model group(n=10),acupuncture-rehabilitation group(n=10)and moxibustion-rehabilitation group(n=10).The latter three groups were induced VaD with 2 vessels occlusion.Eight rats with obvious cognitive impairment were selected from the model groups six weeks after modeling,according to the results of Morris Water Maze;and random eight rats in the sham group were selected.The acupuncture-rehabilitation group accepted scalp acupuncture com‐bined with treadmill training,while the moxibustion-rehabilitation group accepted scalp moxibustion combined with treadmill training.All the rats were tested with Morris Water Maze after four weeks of intervention,and measured the levels of maleic dialdehyde(MDA),superoxide orgotein dismutase(SOD),glutathione(GSH)and glutathione peroxidase reductase(GSH-Px)of hippocampus.Results Compared with the sham group,escape latency increased in the model group,and the number of crossing plat‐forms and the stay time in the target quadrant decreased(P<0.001);while the levels of SOD,GSH and GSH-Px decreased,and the level of MDA increased(P<0.001).Compared with the model group,escape latency de‐creased in the acupuncture-and moxibustion-rehabilitation groups(P<0.001),the number of crossing platforms and the stay time in the target quadrant increased(P<0.001)from the third day;while the levels of SOD,GSH and GSH-Px increased,and the level of MDA decreased(P<0.001);all the indexes imporved more in the moxi‐bustion-rehabilitation group than in the acupuncture-rehabilitation group(P<0.001).Conclusion Scalp moxibustion combined with treadmill training can further relieve the oxidative stress in the hippocam‐pus of VaD rats,to improve the learning and memory function compared with the acupuncture-rehabilitation.