Smad4、Caveolin-1在肺腺癌中的表达及与预后的关系

Expression of Smad4 and Caveolin-1 in lung adenocarcinoma and their correlation with prognosis

目的探讨Smad4、Caveolin-1蛋白在肺腺癌中的表达,分析它们与临床病理因素间的关系及对预后的影响,为分子诊断、预后评估及临床治疗积累数据。方法采用免疫组织化学SP法分别检测130例肺腺癌及癌周正常肺组织中Smad4、Caveolin-1蛋白的表达。结果(1)Smad4、Caveolin-1蛋白在癌周正常肺组织中的表达率分别为90.77%、96.15%,显著高于癌组织中66.15%、41.54%,差异有统计学意义(P<0.05)。(2)腺癌组织中Smad4、Caveolin-1蛋白的表达均与患者性别、年龄、肿块发生部位、有无吸烟及肿块大小无关(P均>0.05),但是与有无淋巴结转移、肿瘤分化程度及TNM分期相关(P均<0.05)。(3)Smad4、Caveolin-1两蛋白在腺癌组织中的表达呈正相关(P<0.05)。(4)Smad4阳性表达组的术后生存时间显著高于阴性表达组,差异有统计学意义(P<0.05),与之相反Caveolin-1阴性表达组的术后生存时间显著高于阳性表达组,差异有统计学意义(P<0.05)。结论Smad4、Caveolin-1蛋白的表达缺失或升高,促进了肿瘤的侵袭性生长和临床进展,提示预后不良,可作为肺腺癌潜在的分子诊断和预后评价指标。

Objective To explore the expression of Smad4 and Caveolin-1 in lung adenocarcinoma,analyze their relationship with clinicopathological factors and their impact on prognosis,and accumulate data for molecular diagnosis,and prognosis evaluation and clinical treatment.Methods Immunohistochemical SP method was used to detect the expression of Smad4 and Caveolin-1 protein in 130 cases of lung adenocarcinoma and normal lung tissue around cancer,respectively.Results(1)The expression rates of Smad4 and Caveolin-1 proteins in normal lung tissue around cancer were 90.77%and 96.15%,respectively,which were significantly higher than 66.15%and 41.54%in cancer tissue,and the difference was statistically significant(P<0.05).(2)The expression of Smad4 and Caveolin-1 in adenocarcinoma tissue was not related to the patient's gender,age,tumor location,smoking,and tumor size(all P>0.05),but was related to lymph node metastasis,tumor differentiation,and TNM stage(all P<0.05).(3)Positive correlation was found between the expressions of Smad4 and Caveolin-1 in lung adenocarcinoma(P<0.05).(4)The postoperative survival time of the Smad4 positive expression group was significantly higher than that of the negative expression group,and the difference was statistically significant(P<0.05).Conclusion The loss or increase of Smad4 and Caveolin-1 protein expression promotes the aggressive growth and clinical progression of the tumor,suggesting poor prognosis,which can be used as a potential molecular diagnosis and prognostic indicator of lung adenocarcinoma.