多肽T9sP对胶质瘤细胞抗肿瘤活性的研究

Study on antitumor activity of T9sP on glioma cell

目的研究来源于E3泛素连接酶TRIM9分子短亚型TRIM9s蛋白的多肽片段T9sP对胶质瘤细胞U87的增殖、迁移和凋亡的影响。方法采用CCK-8法检测T9sP对U87细胞增殖的影响;划痕实验检测多肽对U87细胞迁移的影响;Trans-well实验检测多肽对U87细胞侵袭的影响;Annexin V-FITC/PI双染后使用流式细胞仪检测多肽对U87细胞凋亡的影响。Western Blot法检测多肽对关键信号通路蛋白表达的影响。结果穿膜肽TAT可以促进多肽T9sP进入肿瘤细胞。与对照组相比,多肽T9sP可以显著抑制U87细胞的增殖、迁移和侵袭,促进U87细胞凋亡,T9sP可以显著激活p38信号通路。结论多肽T9sP具有发展为新型抗肿瘤多肽的潜力。

Objective To investigate the antitumor effects of the peptide T9sP which is a short peptide derived from the short form of E3 ubiquitin ligase TRIM9 protein(TRIM9s)on the cell proliferation,migration and apoptosis of glioma cell line U87.Methods CCK-8 assay was performed to determine the effect of T9sP on cell proliferation in U87 cells.The wound healing assay was performed to determine the effect of T9sP on cell migration.Trans-well assay was performed to determine the effect of T9sP on cell invasion in U87 cells.The Annexin V-FITC/PI double staining assay was performed to determine the effect of T9sP on cell apoptosis using flow cytometry.Western blot was performed to determine the effect of T9sP on key proteins.Results The cell penetrating peptide TAT was able to transfer T9sP into cancer cell.Compared with the control group,T9sP was able to inhibit cell proliferation,migration and invasion in U87 cells,and promote cell apoptosis in U87 cells.T9sP was also able to activate the p38 signaling pathway.Conclusion T9sP may have the potential to develop into a novel antitumor peptide.