摘要
目的探讨血清circRNA_100367与鼻咽癌(NPC)患者放疗敏感性及预后的相关性。方法收集2010年9月至2017年10月在鄂州市中心医院头颈外科耳鼻咽喉科接受根治性放疗的NPC患者(NPC组)血清样本222例,另收集同时期体检健康人(对照组)血清样本200例。根据放疗反应性将NPC患者分为放射敏感组和抵抗组。采用实时荧光定量PCR法检测2组血清circRNA_100367水平,并根据其水平将NPC患者分为高表达组和低表达组。单因素和多因素Logistic回归分析血清circRNA_100367水平与NPC患者临床病理特征、放疗反应及预后的关系。结果NPC组血清circRNA_100367水平显著高于对照组(P<0.001),且与患者年龄、TNM分期有关(均P<0.05)。放射敏感组(n=149)血清circRNA_100367水平低于抵抗组(n=73)(P<0.001)。血清circRNA_100367水平是放疗反应的独立预测因子(P<0.05)。血清circRNA_100367用于NPC诊断和放疗反应预测的ROC曲线下面积分别为0.857(95%CI:0.821~0.892)、0.860(95%CI:0.807~0.913)。低表达组(n=148)和高表达组(n=74)患者随访期间无病生存率分别为77.0%、47.3%(χ^(2)=22.647,P<0.001),总生存率分别为81.8%、59.5%(χ^(2)=11.978,P=0.001),无远处转移生存率分别为90.5%、68.9%(χ^(2)=18.694,P<0.001)。结论NPC患者血清中circRNA_100367表达显著上调,且与患者放射抵抗性及预后有关,提示其可能是评估NPC放疗效果和预后的标志物。
Objective To investigate the correlation between serum circRNA_100367 and radiotherapy sensitivity and prognosis in patients with nasopharyngeal carcinoma(NPC).Methods The serum samples were collected from 200 healthy subjects(control group)and 222 patients who received radical radiotherapy for NPC(NPC group)in the department of otorhinolaryngology-head and neck surgery of Ezhou Central Hospital from September 2010 to October 2017.According to the response to radiotherapy,the NPC patients were divided into radiosensitive group(n=149)and radioresistant group(n=73).Serum circRNA_100367 expression was detected by real-time fluorescence quantitative PCR.According to the levels of circRNA_100367,the NPC patients were divided into high expression group(n=74)and low expression group(n=148).The relationships of serum circRNA_100367 levels to clinicopathological features,radiotherapy response and prognosis were evaluated by univariate and multivariate Logistic regression analysis.Results Serum circRNA_100367 levels in NPC group were higher than those in the control group(P<0.001),and were related to patients’age and TNM stage(P<0.05).Furthermore,the levels of circRNA_100367 in radiosensitive group were lower than those in radioresistant group(P<0.001).Serum circRNA_100367 was an independent predictor of radiotherapy response(P<0.05).The area under ROC curve of circRNA_100367 was 0.857(95%CI:0.821-0.892)for NPC diagnosis,and 0.860(95%CI:0.807-0.913)for radiotherapy response prediction.Compared with low circRNA_100367 expression group,high circRNA_100367 expression group had significant increase in the progression-free survival rate(77.0%vs 47.3%,χ^(2)=22.647,P<0.001),overall survival rate(81.8%vs 59.5%,χ^(2)=11.978,P=0.001),and distant metastasis-free survival rate(90.5%vs 68.9%,χ^(2)=18.694,P<0.001).Conclusion Serum circRNA_100367 levels are significantly up-regulated in NPC patients and are correlated with radiation resistance and prognosis,suggesting that circRNA_100367 may be a biomarker to evaluate the efficacy and prognosis of NPC radiotherapy.
作者
皮本元
魏媛媛
佘文胜
PI Ben-yuan;WEI Yuan-yuan;SHE Wen-sheng(Department of ENT,Ezhou Central Hospital,Ezhou 436000,China)
出处
《南昌大学学报(医学版)》
2022年第4期68-74,共7页
Journal of Nanchang University:Medical Sciences
基金
鄂州市科技计划项目(2018sj023)。