摘要
【目的】研究不同剂量PMSG对獭兔的超数排卵效果,并通过胚胎体外观察,探究不同剂量PMSG对獭兔超数排卵后胚胎质量的影响。【方法】选取体质量3~4 kg,7~8月龄18只健康母獭兔,随机分为3组,分别为Ⅰ组(50 IU PMSG+100IU hCG)、Ⅱ组(100 IU PMSG+100 IU hCG)和Ⅲ组(150IU PMSG+100 IU hCG),每组6只,对其进行超数排卵处理。【结果】Ⅰ组平均排卵点数、平均胚胎收集数以及平均可用胚胎数均显著于高于Ⅲ组(P<0.05);Ⅲ组平均卵泡囊肿数显著高于Ⅰ组(P<0.05);Ⅲ组平均充血卵泡数以及胚胎畸形率显著高于Ⅱ组,且Ⅱ组显著高于Ⅰ组(P<0.05)。【结论】用50 IU PMSG+100 IU hCG的外源激素组合处理獭兔,其超排效果较好,可在獭兔生产中参考使用。
【Objective】To study the effect of different doses of PMSG on superovulation in rex rabbits,and on the quality of embryos after superovulation through in vitro observation of embryos.【Method】Eighteen healthy female rex rabbits weighing 3 to 4 kg and 7 to 8 months old were randomly divided into 3 groups(GroupⅠ,50 IU PMSG+100 IU hCG);GroupⅡ,(100 IU PMSG+100 IU hCG)and GroupⅢ,150 IU PMSG+100 IU hCG),6 rats in each group,were treated with superovulation.【Result】The average number of ovulation points,the average number of collected embryos,and the average number of available embryos in GroupⅠwere significantly higher than those in GroupⅢ(P<0.05);The average number of follicular cysts in GroupⅢwas significantly higher than that in GroupⅠ(P<0.05);The average number of congested follicles and embryo malformation rate in GroupⅢwere significantly higher than GroupⅡ,and they were significantly higher than GroupⅠ(P<0.05).【Conclusion】Treatment with the exogenous hormone combination of 50 IU PMSG+100 IU hCG showed a better superovulation effect,which could be used as a reference in the production of rex rabbits.
作者
冉兴荣
伏晓玉
铁雅楠
王欣荣
RAN Xingrong;FU Xiaoyu;TIE Ya'nan;WANG Xinrong(College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,China)
出处
《甘肃农业大学学报》
CAS
CSCD
2022年第3期12-16,24,共6页
Journal of Gansu Agricultural University
基金
甘肃农业大学特色学科建设基金项目(GAU-XKJS-2018-037)。
关键词
獭兔
超数排卵
胚胎发育
rex rabbit
superovulation
embryonic development