集成纳米增强基底的微流控SERS芯片及其致病菌检测

Nano-reinforced substrate integration in SERS microfluidic chip for bacteria detection

针对致病菌快速高效的辨识和检测一直是生命科学、医疗诊断、食品安全和环境监测等领域备受关注的热点。微流控芯片分析技术为细菌等微生物的研究和检测提供了新的、高效的途径和平台,将表面增强拉曼散射(Surface En⁃hanced Raman Scattering,SERS)光谱检测技术与其结合,成为了具有突出优势的致病菌快速鉴别和检测途径。本文针对基于微流控芯片的SERS分析技术及其应用进行综述,首先对各种SERS增强基底材料进行了介绍和性能比较;然后,系统综述了在微流控芯片上集成SERS基底的方法和技术,分别探讨了在微流控通道中注入纳米金属溶胶的外部注入法,在微流控芯片检测区构建固体纳米结构的内嵌法和在微流控通道中原位制备纳米结构基底的原位制作法;最后,对集成SERS检测技术的微流控芯片分析方法在致病菌定性鉴别和定量检测方面的应用进展予以了综述和展望。

The rapid and efficient detection and identification of pathogenic bacteria have long been the focus of many research fields,such as life sciences,medical diagnosis,food safety,and environmental monitoring.Microfluidic chip analysis technology provides efficient methods and platforms for the research and detection of bacteria and other microorganisms.The combination of microfluidic chips and surface-enhanced Raman scattering(SERS)spectroscopy detection technology provides many advantages and allows rapid identification and detection of pathogenic bacteria in biological samples.In this paper,we review the SERS analysis technology and its applications in microfluidic chip analysis.First,we introduce improved substrate materials for SERS and compare their effectiveness.The methods and techniques used to integrate SERS substrates on microfluidic chips are then systematically reviewed.Next,we discuss methods for the external injection of a nano metal sol into the microfluidic channel,embedding a solid nano structure in the microfluidic chip detection area,and the in-situ fabrication of nano structure substrates in the microfluidic channel.Finally,we provide an overview of the progress and future prospects of the application of the microfluidic chip analysis method integrated with SERS detection technology for the identification and quantitative detection of pathogenic bacteria.