基于微流控芯片构建复合力学刺激细胞反应器

Construction of complex mechanical stimulation cell reactor based on microfluidic chip

基于软光刻技术制备了微流控芯片,开发了芯片基材表面修饰技术,有效促进了细胞在微通道内表面的黏附生长,为细胞灌流培养奠定了基础。进一步结合可控的灌流系统和往复伸缩机,构建了对细胞培养体系可同时施加流体剪切力和压力的复合力学刺激细胞反应器。通过在微通道中置入光纤光栅传感器,还可使该反应器能实时监测通道中的压力。该反应器简便小巧,可置于细胞培养箱中,用于研究流体剪切力—压力复合力学刺激对细胞行为的影响。其在复合力学刺激培养细胞或微组织相关研究领域具有潜在的应用价值。

Microfluidic chip is prepared based on soft lithography technology,and surface modification strategy of microfluidic substrate,PDMS,is developed,which effectively promotes the adhesion and growth of cells on the inner surface of microchannel,thus lay a foundation for cell perfusion culture.Then,combines with the controllable perfusion system and reciprocating retractor,a complex mechanical stimulation cell reactor which can simultaneously apply fluid shear stress and compress pressure to the cell culture system is constructed.In addition,by inserting a fiber Bragg grating(FBG)sensor into the microchannel,this cell reactor can also timely sense and transmit the pressure signal in corresponding microchannel.The constructed bioreactor is feasible and small,which can be easily placed into a cell incubator to study the complex mechanical stimulation combined with fluid shear stress and compress pressure on cell behaviors.It has great application potential in the research field of complex mechanical stimulation culture of cell or microtissue.