摘要
近年来太赫兹技术的发展和广泛应用使得太赫兹的生物效应引起了人们的广泛关注。研究连续波0.1 THz对小鼠胚胎成纤维细胞NIH3T3细胞迁移及细胞骨架的影响。采用划痕实验研究0.1 THz辐照对NIH3T3细胞迁移能力的影响,通过荧光染色的方法观察细胞骨架F-actin的形态,通过细胞免疫荧光染色方法检测细胞黏附相关蛋白Paxillin和E-cad-herin的表达水平。划痕实验结果表明,0.1 THz辐照能显著促进NIH3T3细胞迁移,增加细胞增值率;荧光染色结果表明,0.1 THz辐照能够改变NIH3T3细胞F-actin的形态,发生细胞收缩和变形;细胞免疫荧光染色发现,0.1 THz辐照显著降低细胞骨架相关蛋白Paxillin和E-cadherin的表达水平。0.1 THz辐照能够改变成纤维细胞NIH3T3细胞黏附状态,促进细胞迁移及增殖。
With the development and wide application of terahertz technology in recent years,the bio-logical effects of terahertz have attracted extensive attention.To explore the effect of 0.1 THz continuous wave on the migration and cytoskeleton of mouse embryonic fibroblasts NIH3T3cells,the migration abili-ty was measured by wound healing experiment,the morphology of cytoskeleton F-actin was observed by fluorescent staining,and the cell adhesion related proteins Paxillin,Vinculin and E-cadherin expression level were detected by cell immunofluorescence staining.The scratch test results showed that 0.1 THz ir-radiation could significantly promote the migration of NIH3T3cells and increase the cell proliferation rate;the fluorescence staining results showed that 0.1 THz irradiation could change the morphology of F-actin in NIH3T3cells;and the cell immunofluorescence staining showed that 0.1 THz irradiation significantly decreased the expression levels of cytoskeleton-related proteins Paxillin,Vinculin and E-Cadherin.The re-search indicates that 0.1 THz irradiation can change the adhesion state of fibroblast NIH3T3cells and pro-mote cell migration and proliferation.
作者
张琦
尚森
江轶南
卢晓云
ZHANG Qi;SHANG Sen;JIANG Yi-nan;LU Xiao-yun(School of Life Science and Technology of Xi'an Jiaotong University,Institute of Mitochondrial Biomedicine,Key Laboratory of Biomedical Information Engineering,Xi'an710049,China)
出处
《真空电子技术》
2022年第4期31-35,共5页
Vacuum Electronics
基金
国家自然科学基金委员会、中国工程物理研究院联合基金项目(U2030105)。
