红麻HcMYB3基因克隆和表达分析

Gene Cloning and Expression Analysis of HcMYB3 in Kenaf

为探究HcMYB3基因在红麻中的潜在功能和盐胁迫响应规律,研究以红麻品种“中红麻21号”为试验材料,对HcMYB3基因进行同源克隆,并进行生信分析以及盐胁迫下时空表达模式分析。结果表明,HcMYB3基因包含675 bp的开放阅读框,编码224个氨基酸,编码的蛋白质为酸性,具有不稳定、亲水性、不跨膜、不含信号肽的特性,二级结构以无规则卷曲为主,与木槿Hs MYB3亲缘关系最近。HcMYB3基因在中低浓度盐(50、150 mmol/L NaCl)胁迫下显著上调,高浓度盐(250 mmol/L NaCl)胁迫下随时间呈“降—增—降”的趋势。研究为红麻耐盐调控网络的研究提供了理论基础。

To explore the potential function of Hc MYB3 gene in kenaf and its mechanism response to stress,Hc MYB3 gene isolated via homologous cloning method was performed in kenaf cultivar"Zhonghong No.21",followed by bioinformatics analysis and spatio-temporal expression pattern analysis under salt stress.The results showed that the Hc MYB3 gene contained an open reading frame of 675 bp,encoding 224 amino acids,and the secondary structure was dominated by random coil,and the encoded proteins had the characteristics of acidity,unstable,hydrophilicity,non-transmembrane,signal-free peptides.The Hc MYB3 gene was significantly upregulated under the stress of medium and low concentra-tion salts(50 mmol/L,150 mmol/L NaCl),and the trend of"decrease-increase-decrease"was shown over time under high salt concentration of 250 mmol/L NaCl.This study provided theoretical basis for the study of salt-tolerant regulation network of kenaf.