牛樟树提取物促进樟芝深层发酵无性产孢及其应用

Extract of Cinnamomum kanehirae Hay promoting sporulation of Antrodia cinnamomea in submerged fermentation and its application

樟芝(Antrodia cinnamomea)是一种珍稀食药两用蕈菌,具有解酒保肝、抗病毒、抗肿瘤、调节免疫等多种生物活性。基于无性孢子接种的深层发酵技术是樟芝目前最高效、最普遍的人工培养方式,但樟芝深层发酵产孢量低,种子制备成本高,限制了樟芝深层发酵工业化生产效率及效益。为了提高樟芝深层发酵产孢效率,该文首先考察了牛樟树不同溶剂(甲醇、乙酸乙酯、石油醚及水)提取物对樟芝深层发酵无性产孢的影响,发现只有牛樟树水提物能显著促进樟芝无性产孢,且在最佳添加量(60μg/mL)下,可使樟芝产孢量较对照组提高58%左右,同时生物量提高26%左右,三萜产量提高35%左右,多糖产量提高270%左右(即产量是原来的3.7倍);随后,该文将添加牛樟树水提物促进产生的樟芝孢子接种发酵,发现其产孢性能及产胞内多糖性能均显著优于对照组(不添加牛樟树水提物发酵产生的樟芝孢子),其中最大产孢量及胞内多糖产量分别提高了28%及46%左右。该研究成果操作方便、成本低廉、效果显著,较具开发价值及应用前景。

Antrodia cinnamomea(syn Antrodia camphorata and Taiwanofungus camphoratus)is a kind of valuable edible and medici-nal mushroom,and has various biological activities such as hangover alleviation,hepatoprotective,anti-virus,anti-tumor,and regulating immunity.Nevertheless,wild A.cinnamomea fruiting bodies are scarce and in short supply.Thus,the submerged fermentation based on spore inoculation is currently the most efficient artificial cultivation method for A.cinnamomea.However,the submerged fermentation of A.cinnamomea still has some disadvantages,such as low spore production and high cost of inoculum preparation,which largely limit its industrial production efficiency and benefits.The effects of methanol extract,ethyl acetate extract,petroleum ether extract,and water ex-tract of Cinnamomum kanehirae Hay on the sporulation of A.cinnamomea in submerged fermentation were compared,and only the water extract of C.kanehirae(WEC)significantly promoted the spore production of A.cinnamomea.When 60μg/mL of WEC was added to the medium,the spore production increased by 58%compared with the control.At the same time,the biomass and total triterpene increased by 26%and 35%,respectively.The crude intercellular polysaccharide production increased to 3.7 times of the control(i.e.increased by 270%).Lastly,the spores of A.cinnamomea induced by WEC as inoculum were used to study its fermentation performance.The sporulation performance and intracellular polysaccharide production of spores induced by WEC were significantly better than that without WEC inducing(control).Specifically,the maximum spore production and the intracellular polysaccharide production of spores increased by 28%and 46%,respectively.This method has the advantages of convenience,low-cost,and high efficacy,which has great develop-ment value and good application prospects.