不同基因型普通菜豆再生能力研究

Study on Comparison of Regeneration Capacity of Different Varieties of Common Bean

【目的】筛选适宜的普通菜豆再生基因型,为进一步构建普通菜豆遗传转化体系奠定基础。【方法】选取19份普通菜豆品种,将带有子叶节的胚轴作为外植体,在含有2 mg/L 6-BA、0.5 mg/L TDZ、2 mg/L AgNO_(3)的芽诱导培养基上诱导再生芽,统计发芽率、再生芽率以及平均再生芽数,观察再生芽的状态、再生芽速率,综合筛选适宜的再生材料。【结果】细白羊角豆的再生芽率为94.4%,与其他普通菜豆品种差异显著。【结论】初步认为19份材料中细白羊角豆较适合用于构建遗传转化体系。

【Objective】To select the appropriate regeneration genotypes of common beans,to build the construction of the common beans transformation system laid the foundation.【Method】Select 19 common beans,the embryonic axis of the cotyledons was used as explants,regenerative buds were induced on bud-inducing medium containing 2 mg/L 6-BA,0.5 mg/L TDZ and 2 mg/L AgNO_(3).The germination rate,regeneration bud rate and the average number of regenerated buds were counted,and observed the status of the regeneration bud and the regeneration bud rate.Comprehensive screening of suitable regen‐eration materials.【Result】The regeneration bud rate of Xibaiyang Jiaodou was 94.4%,which is significantly different from other common beans.【Conclusion】It is preliminarily concluded that the Xibaiyang Jiaodou is more suitable to construct the genetic transformation system among 19 common beans.